PMID- 10369131
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 67
IP  - 3
DP  - 1999 Apr 15
TI  - Secretion of stem cell factor by alveolar fibroblasts in interstitial lung 
      diseases.
PG  - 229-36
AB  - Sarcoidosis (SA) and diffuse interstitial fibrosis (DIF) are characterized by 
      alveolitis, mast cell hyperplasia and increased fibroblast proliferation. Stem 
      cell factor (SCF) stimulates proliferation of hematopoietic progenitor cells 
      involved in mast and stromal cell interaction. We assessed the role of SCF 
      secreted by alveolar fibroblasts (AFb) in the development of fibrosis of DIF and 
      SA in six patients with SA and six patients with DIF. Bronchoalveolar lavage 
      (BAL) was performed by conventional methods. A total of 500 cells were 
      differentially counted from Giemsa-stained cytopreps. AFb and supernatants were 
      recovered from long-term cultures of BAL cells and from 24 h cultures of 
      confluent AFb. Levels of SCF were measured by ELISA. Alpha actin content of AFb 
      was characterized by immunohistochemistry. The expression of AFb mRNA for 
      IL1-alpha and beta, TGF-beta, IFN-gamma, IL-2, IL-4, IL-5 and IL-6 was determined 
      by RT-PCR. There was a lymphocytic predominance in the SA patients and an 
      increase in neutrophils and eosinophils in DIF. SCF secreted by AFb from DIF was 
      significantly higher than in SA. TNF + IL-1 significantly decreased the secretion 
      of SCF by AFb. There was a positive correlation between SCF levels and the 
      percentage eosinophils but not for metachromatic cells. Alpha-actin expression of 
      AFb in DIF was significantly higher than in SA. Cytokine mRNA was extracted from 
      AFb of two SA and two DIF patients. The profile showed that only in stimulated 
      AFb isolated from the DIF patients can IL-5 transcripts be visualized. In 
      conclusion, AFb can contribute to the onset of fibrosis by secreting SCF and IL-5 
      which, in turn, may recruit eosinophils.
FAU - Fireman, E
AU  - Fireman E
AD  - Department of Pulmonary and Allergic Diseases, Tel-Aviv Sourasky Medical Center, 
      Tel-Aviv, Israel. fireman@post.tau.ac.il
FAU - Kivity, S
AU  - Kivity S
FAU - Shahar, I
AU  - Shahar I
FAU - Reshef, T
AU  - Reshef T
FAU - Mekori, Y A
AU  - Mekori YA
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Stem Cell Factor)
SB  - IM
MH  - Adult
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cytokines/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Pulmonary Alveoli/*metabolism/pathology
MH  - Pulmonary Fibrosis/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoidosis/*metabolism
MH  - Stem Cell Factor/*metabolism
EDAT- 1999/06/16 00:00
MHDA- 1999/06/16 00:01
CRDT- 1999/06/16 00:00
PHST- 1999/06/16 00:00 [pubmed]
PHST- 1999/06/16 00:01 [medline]
PHST- 1999/06/16 00:00 [entrez]
AID - S0165247899000206 [pii]
AID - 10.1016/s0165-2478(99)00020-6 [doi]
PST - ppublish
SO  - Immunol Lett. 1999 Apr 15;67(3):229-36. doi: 10.1016/s0165-2478(99)00020-6.