PMID- 10369274
OWN - NLM
STAT- MEDLINE
DCOM- 19990625
LR  - 20190826
IS  - 0023-852X (Print)
IS  - 0023-852X (Linking)
VI  - 109
IP  - 6
DP  - 1999 Jun
TI  - Clinical application of fluorescence in situ hybridization for chromosome 11q13 
      analysis in head and neck cancer.
PG  - 874-9
AB  - OBJECTIVE: Squamous cell carcinoma of the head and neck (HNSCC) still has one of 
      the lowest 5-year survival rates. Despite advances in diagnosis, treatment, and 
      research, survival rates have not improved in recent years. This report examines 
      the utility of fluorescence in situ hybridization (FISH) in detecting chromosome 
      11q13 amplification in HNSCC and in evaluating the correlation between 11q13 
      amplification and tumor behavior. STUDY DESIGN: This study used FISH to determine 
      the incidence of 11q13 amplification in 20 HNSCCs and 10 normal controls from the 
      same patients. Tumor touch preparations and paraffin-embedded tissues from the 
      same patient samples were used for comparative analysis. Both single and dual 
      color FISH was performed. METHODS: Repetitive chromosome 11 specific alpha 
      satellite DNA probe and chromosome 11q13 specific probe cyclin D1 were used for 
      the FISH analysis. RESULTS: Experiments revealed amplification of chromosome 
      11q13 in three fresh touch preparations. FISH on paraffin tissues showed 
      amplification in two additional samples. Intensity of amplification, as high as 
      20 copies per nucleus, was observed in paraffin preparations, whereas a maximum 
      of only six copies was seen in fresh preparations. Amplification was not detected 
      in any of the normal samples. All five cases with 11q13 amplification had 
      metastases and four of these were from poorly differentiated tumors. In the 
      nonamplified cases, 5 of 15 had metastases and 2 of 15 was poorly differentiated. 
      CONCLUSIONS: The present study indicates that FISH is a useful technique for 
      detecting molecular changes such as amplification of chromosome 11q13 in HNSCC. 
      FISH in paraffin preparations allows for accurate measurement of intensity of 
      amplification and makes it possible for the evaluation of a large collection of 
      archival material. The data also suggest that 11q13 amplification is correlated 
      with poorly differentiated tumors and metastasis. Thus FISH has the potential to 
      be a valuable diagnostic/prognostic tool in head and neck cancers.
FAU - Alavi, S
AU  - Alavi S
AD  - Division of Head and Neck Surgery, VAMC West Los Angeles, UCLA School of 
      Medicine, California 90095-1624, USA.
FAU - Namazie, A
AU  - Namazie A
FAU - Calcaterra, T C
AU  - Calcaterra TC
FAU - Wang, M B
AU  - Wang MB
FAU - Srivatsan, E S
AU  - Srivatsan ES
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Case-Control Studies
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Head and Neck Neoplasms/*genetics/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
EDAT- 1999/06/16 00:00
MHDA- 1999/06/16 00:01
CRDT- 1999/06/16 00:00
PHST- 1999/06/16 00:00 [pubmed]
PHST- 1999/06/16 00:01 [medline]
PHST- 1999/06/16 00:00 [entrez]
AID - 10.1097/00005537-199906000-00006 [doi]
PST - ppublish
SO  - Laryngoscope. 1999 Jun;109(6):874-9. doi: 10.1097/00005537-199906000-00006.