PMID- 10372449
OWN - NLM
STAT- MEDLINE
DCOM- 19990727
LR  - 20191103
IS  - 0188-4409 (Print)
IS  - 0188-4409 (Linking)
VI  - 30
IP  - 2
DP  - 1999 Mar-Apr
TI  - Tumor necrosis factor in peritoneal fluid from asymptomatic infertile women.
PG  - 138-43
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is a cytokine that can be 
      found in the peritoneal fluid (PF) of patients with endometriosis and pelvic 
      inflammatory disease (PID) as a response to inflammatory disorders and 
      infections. The cytotoxic effect of this cytokine could be a factor participating 
      in the pathology of various gynecological diseases, and could also be accountable 
      for the high immunological response and damage to the tubal epithelium. The 
      objective of this study was to establish the presence of TNF-alpha in 
      asymptomatic infertility and its association with various isolated bacteria. 
      METHODS: Ten milliliters of PF were collected from each of 73 patients by means 
      of laparoscopy and cultured in synthetic medium and McCoy cells for the isolation 
      of aerobic and anaerobic bacteria, as well as for Chlamydia trachomatis. The 
      activity of TNF-alpha was determined by means of a bioassay using L-929 cells. 
      RESULTS: Forty-three percent of the PFs showed positive TNF-alpha activity, while 
      the laparoscopic evaluation showed that 32 patients had Fallopian tube occlusion 
      (FTO), 7 had endometriosis, 30 had PID, and 4 had myomas and adhesions. TNF-alpha 
      activity was found to be high in FTO patients (p < 0.05). Positive cultures were 
      found in 50.7% of patients; of these, 31.5% had PID (p < 0.05), and only 20.5% of 
      positive cultures were TNF-alpha positive. Chlamydia trachomatis (16%) was the 
      most frequently isolated bacteria in these patients. CONCLUSIONS: The detection 
      of TNF-alpha could be useful in the diagnosis of active infectious and 
      inflammatory diseases in asymptomatic infertile patients.
FAU - Guerra-Infante, F M
AU  - Guerra-Infante FM
AD  - Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto 
      Politecnico Nacional (ENCB-IPN), Mexico, D.F., Mexico. 
      fguerra@alquimia.encb.ipn.mx
FAU - Flores-Medina, S
AU  - Flores-Medina S
FAU - Lopez-Hurtado, M
AU  - Lopez-Hurtado M
FAU - Zamora-Ruiz, A
AU  - Zamora-Ruiz A
FAU - Sosa Gonzalez, I E
AU  - Sosa Gonzalez IE
FAU - Narcio Reyes, M L
AU  - Narcio Reyes ML
FAU - Villagrana-Zessati, R
AU  - Villagrana-Zessati R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Med Res
JT  - Archives of medical research
JID - 9312706
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Ascitic Fluid/*chemistry
MH  - Female
MH  - Humans
MH  - Infertility, Female/metabolism/*microbiology
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/*analysis
EDAT- 1999/06/18 00:00
MHDA- 1999/06/18 00:01
CRDT- 1999/06/18 00:00
PHST- 1999/06/18 00:00 [pubmed]
PHST- 1999/06/18 00:01 [medline]
PHST- 1999/06/18 00:00 [entrez]
AID - S0188-0128(99)00003-2 [pii]
AID - 10.1016/s0188-0128(99)00003-2 [doi]
PST - ppublish
SO  - Arch Med Res. 1999 Mar-Apr;30(2):138-43. doi: 10.1016/s0188-0128(99)00003-2.