PMID- 10372651
OWN - NLM
STAT- MEDLINE
DCOM- 19990625
LR  - 20220316
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 64
IP  - 23
DP  - 1999
TI  - Blocking of cell proliferation, cytokines production and genes expression 
      following administration of Chinese herbs in the human mesangial cells.
PG  - 2089-99
AB  - In the hope of identifying agents of therapeutic value in immuoglobulin A 
      nephropathy (IgA-N), we tested crude methanol extracts of 15 Chinese herbs for 
      their effect on human mesangial cell proliferation. The results indicated that 4 
      out of the 15 crude extracts inhibited human cells proliferation activated by 
      IL-1beta and IL-6. The extracts and their median inhibitory concentrations were 
      as follows (in microg/ml): Ludwiga octovalvis (MLS-052), 49.9 +/- 1.8; Rhus 
      semialata (MLS-053), 31.2 +/- 1.6; Tabernaemontana divaricata (MLS-054), 50.0 +/- 
      2.1; Amepelopsis brevipedunculata (MLS-059), 42.9 +/- 1.1. These findings 
      indicate that human mesangial cells were most sensitive to MLS-053 treatment. 
      These herbs also decreased interleukin-1beta (IL-1beta) and tumor necrosis 
      factor-alpha (TNF-alpha) production. Moreover, IL- 1beta mRNA expression was 
      inhibited by Rhus semialata (R. semialata; MLS-053). It is unlikely that 
      cytotoxicity was involved, because no cell deaths were observable. We hypothesize 
      that the inhibitory mechanisms of these Chinese herbs may be related to the 
      impairments of gene expression and production of cytokines in human mesangial 
      cells. Plans are underway for the isolation of pure compounds from these Chinese 
      herbs and the elucidation of their mechanisms of action.
FAU - Kuo, Y C
AU  - Kuo YC
AD  - National Research Institute of Chinese Medicine, Veterans Generl Hospital, 
      Department of Biology, Fu Jen University, Taipei, Taiwan, ROC. 
      kuo9111@cma23.nricm.edu.tw
FAU - Sun, C M
AU  - Sun CM
FAU - Tsai, W J
AU  - Tsai WJ
FAU - Ou, J C
AU  - Ou JC
FAU - Chen, W P
AU  - Chen WP
FAU - Lin, C Y
AU  - Lin CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-1)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cell Survival/drug effects
MH  - Cytokines/*biosynthesis/genetics
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Gene Expression/drug effects
MH  - Glomerular Mesangium/cytology/*drug effects
MH  - Humans
MH  - Interleukin-1/biosynthesis/genetics
MH  - Kidney Diseases/drug therapy/pathology
MH  - Plant Extracts/pharmacology/therapeutic use
MH  - RNA, Messenger/drug effects/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 1999/06/18 00:00
MHDA- 1999/06/18 00:01
CRDT- 1999/06/18 00:00
PHST- 1999/06/18 00:00 [pubmed]
PHST- 1999/06/18 00:01 [medline]
PHST- 1999/06/18 00:00 [entrez]
AID - S0024320599001587 [pii]
AID - 10.1016/s0024-3205(99)00158-7 [doi]
PST - ppublish
SO  - Life Sci. 1999;64(23):2089-99. doi: 10.1016/s0024-3205(99)00158-7.