PMID- 10374707
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20190921
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 41
IP  - 4
DP  - 1999 Apr
TI  - Quantitative assessment of human leukocyte antigen-G protein in amniotic fluid by 
      a double-determinant enzyme-linked immunosorbent assay using anti-human leukocyte 
      antigen-G-specific antibody '87G'.
PG  - 293-5
AB  - PROBLEM: The objective of this study was to establish an enzyme-linked 
      immunosorbent assay (ELISA) system, in an attempt to quantify the amount of human 
      leukocyte antigen (HLA)-G protein in amniotic fluid. METHOD OF STUDY: We 
      established a double-determinant ELISA system using the anti-HLA-G specific mouse 
      monoclonal antibody '87G' as a capture antibody and the horseradish-peroxidase 
      labeled rabbit anti-human beta2-microglobulin antibody as a detection antibody. 
      We then measured the concentration of HLA-G protein in amniotic fluid samples 
      from nine normal second-trimester pregnant women and in serum samples from eight 
      normal males. RESULTS AND CONCLUSION: HLA-G protein was detected in amniotic 
      fluid at a concentration of 275 ng/ml (197-343 ng/ml) (median value and 95% 
      confident range), whereas the concentration of HLA-G protein in male serum was 
      below the minimum detection level.
FAU - Hamai, Y
AU  - Hamai Y
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, University of 
      Tokyo, Japan.
FAU - Fujii, T
AU  - Fujii T
FAU - Miki, A
AU  - Miki A
FAU - Geraghty, D E
AU  - Geraghty DE
FAU - Harada, I
AU  - Harada I
FAU - Takai, Y
AU  - Takai Y
FAU - Kozuma, S
AU  - Kozuma S
FAU - Tsutsumi, O
AU  - Tsutsumi O
FAU - Taketani, Y
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adult
MH  - Amniotic Fluid/*immunology
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Female
MH  - HLA Antigens/*analysis/blood/immunology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*analysis/blood/immunology
MH  - Humans
MH  - Male
MH  - Mice
MH  - Pregnancy
MH  - Pregnancy Trimester, Second
MH  - Trophoblasts/immunology
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - 10.1111/j.1600-0897.1999.tb00441.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1999 Apr;41(4):293-5. doi: 
      10.1111/j.1600-0897.1999.tb00441.x.