PMID- 10374781
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 30
IP  - 6
DP  - 1999 Jun
TI  - Trisomy 3 in gastric lymphomas of extranodal marginal zone B-cell 
      (mucosa-associated lymphoid tissue) origin demonstrated by FISH in intact 
      paraffin tissue sections.
PG  - 706-11
AB  - Some reports have indicated that trisomy 3 represents a characteristic 
      chromosomal abnormality found in lymphomas arising in mucosa-associated lymphoid 
      tissues (MALT)/extranodal marginal zone B-cells (MZBC). Traditional cytogenetic 
      analysis of metaphase preparations is cumbersome and not always possible, 
      especially in those situations in which the diagnosis in not suspected before a 
      biopsy. Our aim is to use a relatively simple method to evaluate trisomy 3 in 
      paraffin-embedded, formalin-fixed tissue, using fluorescence in situ 
      hybridization (FISH) on intact tissue sections. Formalin-fixed, paraffin-embedded 
      archival tissues from 30 cases (27 lymphoma and 3 chronic gastritis cases) were 
      hybridized with a chromosome 3 specific alpha-satellite probe (ONCOR, 
      Gaithersburg, MD). Three of four cases of gastric MZBC/MALT lymphoma revealed 
      trisomy 3. Ten cases of lymphoma of possible or probable MZBC origin were 
      examined, and four revealed trisomy 3. Five of 13 non-MZBC lymphomas revealed 
      trisomy 3. None of the chronic gastritis cases nor normal tonsil cases revealed 
      trisomy 3. Our results, using a different methodological approach, confirm the 
      findings of others that trisomy 3 is an abnormality found in a significant 
      proportion of lymphomas of MZBC origin. Our approach also makes possible 
      interphase cytogenetic analysis (by FISH) of routinely processed formalin-fixed, 
      paraffin-embedded tissues, without the need to disaggregate cells. It thus may 
      facilitate genetic analysis on specimens previously deemed unsuitable for such 
      analysis, particularly when tissue quantity is limited.
FAU - Blanco, R
AU  - Blanco R
AD  - University of Cincinnati Medical College, OH, USA.
FAU - Lyda, M
AU  - Lyda M
FAU - Davis, B
AU  - Davis B
FAU - Kraus, M
AU  - Kraus M
FAU - Fenoglio-Preiser, C
AU  - Fenoglio-Preiser C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 3
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell, Marginal Zone/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Stomach Neoplasms/*genetics/pathology
MH  - *Trisomy
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S0046-8177(99)90098-9 [pii]
AID - 10.1016/s0046-8177(99)90098-9 [doi]
PST - ppublish
SO  - Hum Pathol. 1999 Jun;30(6):706-11. doi: 10.1016/s0046-8177(99)90098-9.