PMID- 10374857
OWN - NLM
STAT- MEDLINE
DCOM- 19990707
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 23
IP  - 5
DP  - 1999 May
TI  - Development of a simple, blood based lymphoproliferation assay to assess the 
      clinical status of patients with acute lymphoblastic leukemia.
PG  - 433-9
AB  - Although childhood acute lymphoblastic leukemia (ALL) is highly responsive to 
      chemotherapy, reliable techniques are needed to determine treatment outcome and 
      predict relapse. Employing a 9-O-acetyl sialic acid binding lectin, ATN(H), we 
      have identified two 9-O-acetylated sialogycoconjugates (9-OAcSGs) as novel 
      biomarkers expressed selectively on leukemic blasts of ALL patients. Presently, 
      we report a non-invasive, blood based lymphoproliferation assay, which employs 
      the maximal lymphoproliferative dose of ATN(H) (MLD) to assess the status of 
      9-OAcSGs with progressive therapy. A low MLD (0.18 +/- 0.01 microg) in untreated 
      patients reflects increased expression of 9-OAcSGs which decline following 
      therapy (MLD = 2.10 +/- 0.60 microg), persist during maintenance therapy (MLD = 
      4.50 +/- 1.60 microg)/follow-up (MLD = 5.50 +/- 0.85 microg) and are re-induced 
      with relapse (MLD = 0.25 +/- 0.01 microg). Since the assay detects lymphoblasts 
      with a sensitivity of 10(-4), shows no cross-reactivity with other hematological 
      disorders (n = 48) and has been tested in 212 patients, it meets clinical 
      consideration.
FAU - Sinha, D
AU  - Sinha D
AD  - Indian Institute of Chemical Biology, Calcutta.
FAU - Mandal, C
AU  - Mandal C
FAU - Bhattacharya, D K
AU  - Bhattacharya DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitogens)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Cross Reactions
MH  - Evaluation Studies as Topic
MH  - Female
MH  - Glycoproteins/drug effects
MH  - Humans
MH  - Lymphocytes/pathology
MH  - Lymphoproliferative Disorders/*blood
MH  - Male
MH  - Membrane Proteins/drug effects
MH  - Mitogens/pharmacology
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood/*drug therapy
MH  - Prognosis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Stem Cells/pathology
MH  - Treatment Outcome
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S0145-2126(98)00184-2 [pii]
AID - 10.1016/s0145-2126(98)00184-2 [doi]
PST - ppublish
SO  - Leuk Res. 1999 May;23(5):433-9. doi: 10.1016/s0145-2126(98)00184-2.