PMID- 10375018
OWN - NLM
STAT- MEDLINE
DCOM- 19990820
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 149
IP  - 1-2
DP  - 1999 Mar 25
TI  - Ligand regulation of juvenile hormone binding protein mRNA in mutant Manduca 
      sexta.
PG  - 61-9
AB  - Insect hemolymph juvenile hormone binding protein (hJHBP) regulates peripheral 
      titers of its ligands, the juvenile hormones. In larvae of the black (bl) strain 
      of the tobacco hornworm, Manduca sexta, treatment with small doses of juvenile 
      hormone I (JH I) can also regulate titers of hJHBP. To further investigate this 
      regulation, responsiveness of hJHBP mRNA expression to JH I was characterized in 
      vivo. RNA analyzes revealed that transcript levels in fat body, the site of hJHBP 
      synthesis, increased fivefold within several hours of treatment with 
      physiological doses of hormone and remained elevated for approximately 16 h. 
      Sensitivity to JH treatment was found to vary temporally. To ensure transcript 
      identity, a wild-type cDNA clone and a bl RT-PCR fragment were sequenced and 
      found to be 99% homologous. Together, these results suggest that JH participates 
      in regulating expression of its transport protein in bl larvae by modifying the 
      in vivo abundance of hJHBP's mRNA transcript.
FAU - Orth, A P
AU  - Orth AP
AD  - Department of Entomology, University of Wisconsin-Madison, 53706, USA.
FAU - Lan, Q
AU  - Lan Q
FAU - Goodman, W G
AU  - Goodman WG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Ecdysteroids)
RN  - 0 (Insect Hormones)
RN  - 0 (Insect Proteins)
RN  - 0 (Juvenile Hormones)
RN  - 0 (Ligands)
RN  - 0 (RNA, Messenger)
RN  - 0 (Steroids)
RN  - 0 (juvenile hormone-binding protein, insect)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - DNA, Complementary/genetics
MH  - Ecdysteroids
MH  - Fat Body/metabolism
MH  - Insect Hormones/metabolism
MH  - *Insect Proteins
MH  - Juvenile Hormones/*metabolism
MH  - Larva/metabolism
MH  - Ligands
MH  - Manduca/*genetics/growth & development/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - RNA, Messenger/*genetics/*metabolism
MH  - Steroids/metabolism
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S0303-7207(98)00258-5 [pii]
AID - 10.1016/s0303-7207(98)00258-5 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1999 Mar 25;149(1-2):61-9. doi: 
      10.1016/s0303-7207(98)00258-5.