PMID- 10375201
OWN - NLM
STAT- MEDLINE
DCOM- 19990820
LR  - 20190905
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 24
IP  - 6
DP  - 1999 Jun
TI  - Trabecular remodeling processes in the ovariectomized rat: modified node-strut 
      analysis.
PG  - 591-6
AB  - The purpose of this study was to evaluate the trabecular bone remodeling 
      processes in ovariectomized rats, focusing on diminishing trabecular 
      connectivity. We used modified node-strut analysis defining three areas in the 
      trabecular surfaces for the three-dimensional understanding of trabecular 
      resorption derived from two-dimensional conventional sections, in addition to 
      conventional bone histomorphometry and node-strut analysis. Seven-month-old 
      female Wistar rats were used and treated with bilateral ovariectomy (ovx) and 
      sham operation. Six rats in each group were examined at 4 and 8 weeks. We 
      prepared undecalcified sections from the left tibiae with Villanueva bone and 
      Goldner stains. We divided the trabecular bone surfaces (BS) into three areas: 
      node (Nd), terminus (Tm), and strut (St), and measured the bone resorption and 
      formation parameters, including eroded surface (ES), osteoclast surface (Oc.S), 
      osteoid surface (OS), and double-labeled surface (dLS) in each defined area. In 
      conventional bone histomorphometry, the ovx group showed high turnover osteopenia 
      compared with the sham operation group. In node-strut analysis, the ovx group 
      showed significantly lower values for node-related parameters than did the sham 
      operation group. In the modified node-strut analysis, bone resorption parameters 
      in the ovx group showed significantly higher values, particularly for strut and 
      terminus-eroded surfaces (StES/BS, TmES/BS), and for each area of osteoclast 
      surface (NdOc.S/BS, TmOc.S/BS, and StOc.S/BS) compared with the sham operation 
      group. Bone formation parameters in the ovx group also showed significantly 
      higher values, particularly for strut and terminus osteoid surfaces (TmOS/BS, 
      StOS/BS), and for each area of double-labeled surface (NddLS/BS, TmdLS/BS, and 
      StdLS/BS) compared with the sham operation group at 4 weeks. At 8 weeks, each 
      area of bone formation parameter in the ovx group showed significantly higher 
      values than that in the sham operation group. These results suggest that in the 
      ovx group, the trabecular plates became perforated and the perforative cavities 
      progressively enlarged, and/or the edges of plates were eroded regardless of 
      elevated bone formation, resulting in diminished trabecular connectivity, and the 
      node area might not be influenced relatively by bone remodeling in the early 
      resorption.
FAU - Abe, T
AU  - Abe T
AD  - Department of Orthopedic Surgery, Akita University School of Medicine, Japan.
FAU - Sato, K
AU  - Sato K
FAU - Miyakoshi, N
AU  - Miyakoshi N
FAU - Kudo, T
AU  - Kudo T
FAU - Tamura, Y
AU  - Tamura Y
FAU - Tsuchida, T
AU  - Tsuchida T
FAU - Kasukawa, Y
AU  - Kasukawa Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
SB  - IM
MH  - Animals
MH  - Bone Remodeling/*physiology
MH  - Bone Resorption/etiology/pathology
MH  - Bone and Bones/pathology
MH  - Female
MH  - Microscopy, Electron, Scanning
MH  - Models, Anatomic
MH  - Ovariectomy/adverse effects
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S8756-3282(99)00080-0 [pii]
AID - 10.1016/s8756-3282(99)00080-0 [doi]
PST - ppublish
SO  - Bone. 1999 Jun;24(6):591-6. doi: 10.1016/s8756-3282(99)00080-0.