PMID- 10375400
OWN - NLM
STAT- MEDLINE
DCOM- 19990715
LR  - 20211203
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 367
IP  - 1
DP  - 1999 Jul 1
TI  - Phosphatidylinositol 3-kinase regulates differentiation of H9c2 cardiomyoblasts 
      mainly through the protein kinase B/Akt-independent pathway.
PG  - 67-73
AB  - Phosphatidylinositol 3-kinase (PI3-kinase) is known to be a crucial regulator of 
      muscle differentiation. However, its downstream pathway for this function is 
      quite obscure. In this experiment we demonstrated the regulatory mechanism of the 
      differentiation of H9c2 cardiomyoblasts, focusing on PI3-kinase, protein kinase 
      B/Akt (PKB/Akt) and p42/44 mitogen-activated protein kinase (p42/44 MAPK). When 
      H9c2 cells stably transfected with a constitutively active p110 (H9c2-p110*), a 
      constitutively active PKB/Akt (H9c2-Akt), and an empty vector (H9c2-con) were 
      induced to differentiate, H9c2-p110* cells differentiated fastest, followed by 
      H9c2-Akt cells. H9c2-con cells differentiated at the slowest rate. Consistent 
      with this result, LY294002 completely blocked differentiation of all these 
      transfected cell lines, whereas PD098059 had no effect on their differentiation. 
      When H9c2-p110* cells were transiently transfected with a dominant negative form 
      of PKB/Akt, differentiation was not affected. Taken together, we concluded that 
      PI3-kinase, but not p42/44 MAPK, regulates differentiation of H9c2 
      cardiomyoblasts mainly through the PKB/Akt-independent pathway.
CI  - Copyright 1999 Academic Press.
FAU - Kim, J M
AU  - Kim JM
AD  - School of Natural Science, Hanyang University, Seoul, 133-791, Korea.
FAU - Yoon, M Y
AU  - Yoon MY
FAU - Kim, J
AU  - Kim J
FAU - Kim, S S
AU  - Kim SS
FAU - Kang, I
AU  - Kang I
FAU - Ha, J
AU  - Ha J
FAU - Kim, S S
AU  - Kim SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Chromones)
RN  - 0 (Flavonoids)
RN  - 0 (Insulin)
RN  - 0 (Morpholines)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - EC 2.7.11.1 (Akt1 protein, rat)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Size/drug effects
MH  - Chromones/pharmacology
MH  - Flavonoids/pharmacology
MH  - Genes, Dominant/genetics
MH  - Heart/drug effects
MH  - Insulin/pharmacology
MH  - Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism
MH  - Morpholines/pharmacology
MH  - Mutation
MH  - Myocardium/*cytology/metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics/*metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation/drug effects
MH  - Precipitin Tests
MH  - *Protein Serine-Threonine Kinases
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-akt
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - *Signal Transduction/drug effects
MH  - Transfection
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S0003-9861(99)91232-8 [pii]
AID - 10.1006/abbi.1999.1232 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 1999 Jul 1;367(1):67-73. doi: 10.1006/abbi.1999.1232.