PMID- 10375860
OWN - NLM
STAT- MEDLINE
DCOM- 19990720
LR  - 20061115
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 26
IP  - 2
DP  - 1999
TI  - [Aneuploidy detection by multi-color fluorescence in situ hybridization in mouse 
      sperm].
PG  - 112-8
AB  - Multi-color fluorescence in situ hybridization (FISH) was employed to evaluate 
      the frequency of aneuploid sperm in young adult mice after treated by 
      2-(4'-thiazolyl) benzimidazole or thiabendazole (TB). The animals were given TB 
      (100 200 300 mg/kg) by oral incubation daily for 11 days, and sacrificed 22 days 
      after the last treatment of TB. Three-chromosome FISH was applied to determine 
      the frequencies of diploid, disomic and nullisomic sperm with DNA probes specific 
      for the chromosomes X, Y and 8. The aneuploid frequencies were not significantly 
      different between the sperm in 100 mg/kg group and solvent control. The 
      frequencies aneuploid and diploid sperm in middle dose group were significantly 
      higher than the solvent control (P < 0.001-0.05). There were no significant 
      aneuploid frequency increases were found in 300 mg/kg group excepting the 
      frequency of diploid sperm. It is conclude that TB is a potential germinal 
      aneugen in mammalian, which may block the cleavage during the meiosis. Epididymal 
      sperm aneuploidy can be reliably determined by sperm FISH analysis in mice.
FAU - Wang, X
AU  - Wang X
AD  - Department of Life Sciences, Yunnan Normal University, Kunming.
FAU - Schmid, T
AU  - Schmid T
FAU - Adler, I D
AU  - Adler ID
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Spermatozoa/*ultrastructure
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 1999;26(2):112-8.