PMID- 10376809
OWN - NLM
STAT- MEDLINE
DCOM- 19990812
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 11
IP  - 5
DP  - 1999 May
TI  - Intracellular free calcium regulates the onset of the respiratory burst of human 
      neutrophils activated by phorbol myristate acetate.
PG  - 355-60
AB  - Thapsigargin was used to study the regulation of different static calcium level 
      ([Ca2+]i) on the respiratory hurst of human neutrophils stimulated with phorbol 
      myristate acetate (PMA). The result showed that the onset time of the respiratory 
      hurst was obviously reduced by elevation of static [Ca2+]i but is still much 
      longer than that stimulated with N-formylmethionylleucylphenylalanine (fMLP). To 
      find the reason, the onset times of the respiratory burst stimulated with fMLP, 
      1,2-dioctanoyl-sn-glycerol (DiC8), and PMA were determined at different static 
      [Ca2+]i. It turns out that although DiC8 was unable to induce the respiratory 
      burst at low [Ca2+], the onset time of DiC8-stimulated response at high [Ca2+]i 
      was almost the same as that stimulated with fMLP. The study revealed that the 
      fast onset of the fMLP-stimulated respiratory burst in comparison with 
      PMA-stimulated response is not only due to the transient rise of [Ca2+]i, but is 
      also due to the higher efficiency of diacylglycerol (DAG) in activating protein 
      kinase c (PKC). The determining step in governing the onset of a respiratory 
      burst is the activation of PKC.
FAU - Hu, T H
AU  - Hu TH
AD  - Institute of Biophysics, Chinese Academy of Sciences, Beijing, PR China.
FAU - Bei, L
AU  - Bei L
FAU - Qian, Z M
AU  - Qian ZM
FAU - Shen, X
AU  - Shen X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Diglycerides)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Mitogens)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 67526-95-8 (Thapsigargin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - Cells, Cultured
MH  - Diglycerides/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Humans
MH  - Intracellular Fluid/metabolism
MH  - Mitogens/*metabolism/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophil Activation
MH  - Neutrophils/drug effects/*metabolism
MH  - *Respiratory Burst/drug effects
MH  - Tetradecanoylphorbol Acetate/*metabolism/pharmacology
MH  - Thapsigargin/pharmacology
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S0898-6568(99)00007-8 [pii]
AID - 10.1016/s0898-6568(99)00007-8 [doi]
PST - ppublish
SO  - Cell Signal. 1999 May;11(5):355-60. doi: 10.1016/s0898-6568(99)00007-8.