PMID- 10376999
OWN - NLM
STAT- MEDLINE
DCOM- 19991005
LR  - 20190822
IS  - 0166-6851 (Print)
IS  - 0166-6851 (Linking)
VI  - 100
IP  - 1
DP  - 1999 May 15
TI  - Identification of heparin as a ligand for the A-domain of Plasmodium falciparum 
      thrombospondin-related adhesion protein.
PG  - 111-24
AB  - Thrombospondin-related adhesion protein (TRAP) is a Plasmodium falciparum 
      transmembrane protein that is expressed within the micronemes of sporozoites, and 
      is implicated in host cell invasion and motility. Contained within the 
      extracellular region of TRAP is an A-domain, a module found in a number of 
      membrane, plasma and matrix proteins, that is often involved in ligand 
      recognition. In order to determine the role of the TRAP A-domain, it has been 
      expressed as a glutathione S-transferase fusion protein and its ligand binding 
      compared with that of other characterised glutathione S-transferase A-domain 
      fusion proteins. Using a solid phase assay to screen for binding to known 
      A-domain ligands, the TRAP A-domain was found to bind heparin. Binding to heparin 
      appeared to be specific as it was saturable, and was inhibited by soluble 
      heparin, fucoidan and dextran sulfate, but not by other negatively charged 
      sulfated glycosaminoglycans such as chondroitin sulfates. Furthermore, unlike 
      some A-domain ligand interactions, the A-domain of both TRAP and the leukocyte 
      integrin, Mac-1, bound to heparin in the absence of divalent cations. It has been 
      shown previously that another domain within TRAP, which is homologous to region 
      II-plus of circumsporozoite protein, binds to sulfatide and to heparan sulfate on 
      the immortalised hepatocyte line HepG2. The TRAP A-domain also bound to sulfatide 
      and to HepG2 cells. Thus the A-domain shares certain binding properties already 
      attributed to the region II-plus-like domain of TRAP, and may contribute to the 
      binding of TRAP to heparan sulfate on hepatocytes.
FAU - McCormick, C J
AU  - McCormick CJ
AD  - School of Biology, University of Leeds, UK. bgycjm@leeds.ac.uk
FAU - Tuckwell, D S
AU  - Tuckwell DS
FAU - Crisanti, A
AU  - Crisanti A
FAU - Humphries, M J
AU  - Humphries MJ
FAU - Hollingdale, M R
AU  - Hollingdale MR
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Ligands)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 120300-02-9 (thrombospondin-related adhesive protein, protozoan)
RN  - 9005-49-6 (Heparin)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Glutathione Transferase/genetics/metabolism
MH  - Heparin/*metabolism
MH  - Humans
MH  - Ligands
MH  - Macrophage-1 Antigen/metabolism
MH  - Plasmodium falciparum/genetics/growth & development/*metabolism
MH  - Protozoan Proteins/chemistry/genetics/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
AID - S0166-6851(99)00052-3 [pii]
AID - 10.1016/s0166-6851(99)00052-3 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 1999 May 15;100(1):111-24. doi: 
      10.1016/s0166-6851(99)00052-3.