PMID- 10377180
OWN - NLM
STAT- MEDLINE
DCOM- 19990707
LR  - 20181201
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 103
IP  - 12
DP  - 1999 Jun
TI  - SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast 
      cells.
PG  - 1737-43
AB  - SLP-76 is an adapter protein expressed in T cells and myeloid cells that is a 
      substrate for ZAP-70 and Syk. SLP-76-deficient mice exhibit a profound block in 
      T-cell development. We found that although SLP-76 is expressed in mouse mast 
      cells, SLP-76(-/-) mice have normal numbers of mast cells in their skin and 
      bronchi. SLP-76(-/-) mice are resistant to IgE-mediated passive anaphylaxis. 
      SLP-76(-/-) mice sensitized with IgE anti-dinitrophenyl (DNP) and then challenged 
      with DNP-HSA developed only mild and transient tachycardia, failed to increase 
      their plasma histamine level, and all survived the antigen challenge. Bone 
      marrow-derived mast cells (BMMCs) from SLP76(-/-) mice failed to release 
      beta-hexosaminidase and to secrete IL-6 after FcepsilonRI cross-linking. Tyrosine 
      phosphorylation of phospholipase C-gamma1 (but not of Syk) and calcium 
      mobilization in response to IgE cross-linking were reduced in SLP-76-deficient 
      BMMCs. These results suggest that SLP-76 plays an important role in 
      FcepsilonRI-mediated signaling in mast cells.
FAU - Pivniouk, V I
AU  - Pivniouk VI
AD  - Division of Immunology, Children's Hospital, Harvard Medical School, Boston, 
      Massachusetts 02115, USA.
FAU - Martin, T R
AU  - Martin TR
FAU - Lu-Kuo, J M
AU  - Lu-Kuo JM
FAU - Katz, H R
AU  - Katz HR
FAU - Oettgen, H C
AU  - Oettgen HC
FAU - Geha, R S
AU  - Geha RS
LA  - eng
GR  - P01 AI035714/AI/NIAID NIH HHS/United States
GR  - R01 AI041144/AI/NIAID NIH HHS/United States
GR  - AI-35714/AI/NIAID NIH HHS/United States
GR  - AI-41144/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Cytokines)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, IgE)
RN  - 0 (SLP-76 signal Transducing adaptor proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Adoptive Transfer
MH  - Animals
MH  - Binding Sites/genetics
MH  - Bone Marrow Cells/metabolism/physiology
MH  - Cell Degranulation/genetics
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Immunoglobulin E/administration & dosage/physiology
MH  - Mast Cells/*metabolism/physiology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Knockout
MH  - Passive Cutaneous Anaphylaxis
MH  - Phosphoproteins/biosynthesis/*deficiency/*genetics
MH  - Receptors, IgE/genetics/immunology/*physiology
MH  - Signal Transduction/*genetics
PMC - PMC408386
EDAT- 1999/06/22 00:00
MHDA- 1999/06/22 00:01
PMCR- 1999/06/15
CRDT- 1999/06/22 00:00
PHST- 1999/06/22 00:00 [pubmed]
PHST- 1999/06/22 00:01 [medline]
PHST- 1999/06/22 00:00 [entrez]
PHST- 1999/06/15 00:00 [pmc-release]
AID - 06338 [pii]
PST - ppublish
SO  - J Clin Invest. 1999 Jun;103(12):1737-43.