PMID- 10377991
OWN - NLM
STAT- MEDLINE
DCOM- 19990726
LR  - 20240109
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 18
IP  - 3
DP  - 1999 May
TI  - Tobacco retinoblastoma-related protein phosphorylated by a distinct 
      cyclin-dependent kinase complex with Cdc2/cyclin D in vitro.
PG  - 243-52
AB  - The retinoblastoma (Rb) protein was originally identified as a product of a 
      tumour suppressor gene that plays a pivotal role in regulating both the cell 
      cycle and differentiation in mammals. The growth-suppressive activity of Rb is 
      regulated by phosphorylation with cyclin-dependent kinase (CDK), and inactivation 
      of the Rb function is one of the critical steps for transition from the G1 to the 
      S phase. We report here the cloning of a cDNA (NtRb1) from Nicotiana tabacum 
      which encodes a Rb-related protein, and show that this gene is expressed in all 
      the organs examined at the mRNA level. We have demonstrated that NtRb1 interacts 
      with tobacco cyclin D by using yeast two-hybrid and in vitro binding assays. In 
      mammals, cyclin D can assemble with CDK4 and CDK6, but not with Cdc2, to form 
      active complexes. Surprisingly, tobacco cyclin D and Cdc2 proteins can form a 
      complex in insect cells, which is able to phosphorylate tobacco Rb-related 
      protein in vitro. Using immunoprecipitation with the anti-cyclin D anti-body, 
      cyclin D can be found in a complex with Cdc2 in suspension-cultured tobacco BY-2 
      cells. These results suggest that the cdc2 gene modulates the cell cycle through 
      the phosphorylation of Rb-related protein by forming an active complex with 
      cyclin D in plants.
FAU - Nakagami, H
AU  - Nakagami H
AD  - Graduate School of Biological Sciences, Nara Institute of Science and Technology 
      (NAIST), Japan.
FAU - Sekine, M
AU  - Sekine M
FAU - Murakami, H
AU  - Murakami H
FAU - Shinmyo, A
AU  - Shinmyo A
LA  - eng
SI  - GENBANK/AB015221
SI  - GENBANK/AB015222
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin D)
RN  - 0 (Cyclins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Plant Proteins)
RN  - 0 (RRB1 protein, Zea mays)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CDC2 Protein Kinase/metabolism
MH  - *Cell Cycle Proteins
MH  - Cyclin D
MH  - Cyclin-Dependent Kinases/*metabolism
MH  - Cyclins/metabolism
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Gene Expression
MH  - Genes, Plant
MH  - Humans
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Plant Proteins/*genetics/*metabolism
MH  - *Plants, Toxic
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Retinoblastoma Protein/*genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - Nicotiana/*genetics/*metabolism
EDAT- 1999/06/23 00:00
MHDA- 1999/06/23 00:01
CRDT- 1999/06/23 00:00
PHST- 1999/06/23 00:00 [pubmed]
PHST- 1999/06/23 00:01 [medline]
PHST- 1999/06/23 00:00 [entrez]
AID - 10.1046/j.1365-313x.1999.00449.x [doi]
PST - ppublish
SO  - Plant J. 1999 May;18(3):243-52. doi: 10.1046/j.1365-313x.1999.00449.x.