PMID- 10378780
OWN - NLM
STAT- MEDLINE
DCOM- 19990701
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 138
IP  - 1-2
DP  - 1999 Apr 26
TI  - Flow cytometric DNA analysis and chromosomal aberrations in malignant 
      glioblastomas.
PG  - 101-6
AB  - In this study we combined flow cytometry with fluorescence in situ hybridization 
      to detect numerical aberrations in chromosomes. Fifty-nine human malignant 
      gliomas were examined by flow cytometry for DNA-content and cell cycle analysis 
      and for numerical aberrations of chromosome 1 by in situ hybridization using a 
      chromosome specific centromere probe. Of the gliomas analysed, 42% were diploid 
      and 58% showed aneuploid tumour cell populations. The DNA index was heterogeneous 
      ranging from 1.0 to 2.3. The S-phase analysis showed proliferation activity from 
      a very low range of 0.7% up to 17.0%. In general, diploid gliomas exhibited a 
      lower S-phase activity than aneuploid gliomas. Of the aneuploid gliomas, 15% 
      showed a peridiploid pattern with a DNA index mean of 1.1. In these peridiploid 
      tumours a trisomy of chromosome 1 could be detected by fluorescence in situ 
      hybridization (FISH). The frequency of trisomic chromosome 1 in malignant gliomas 
      reflects a very slight increase in DNA index from diploid to peridiploid (DNA 
      index 1.1). Comparison of chromosome numbers and DNA content gave good 
      correlation. Also important, the results reflects the cell cycle, specifically 
      the extent of S-phase activity. In general, cell proliferation of diploid and 
      peridiploid gliomas is much less than in higher aneuploid gliomas. The analysis 
      of DNA content may thus yield results with respect to the biological behaviour of 
      tumours in general.
FAU - Ehemann, V
AU  - Ehemann V
AD  - Institute of Pathology, University of Heidelberg, Germany.
FAU - Hashemi, B
AU  - Hashemi B
FAU - Lange, A
AU  - Lange A
FAU - Otto, H F
AU  - Otto HF
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/*analysis
MH  - *Flow Cytometry
MH  - Glioblastoma/*genetics
MH  - Humans
MH  - S Phase
MH  - Tumor Cells, Cultured
EDAT- 1999/06/23 00:00
MHDA- 1999/06/23 00:01
CRDT- 1999/06/23 00:00
PHST- 1999/06/23 00:00 [pubmed]
PHST- 1999/06/23 00:01 [medline]
PHST- 1999/06/23 00:00 [entrez]
AID - S0304-3835(98)00383-8 [pii]
AID - 10.1016/s0304-3835(98)00383-8 [doi]
PST - ppublish
SO  - Cancer Lett. 1999 Apr 26;138(1-2):101-6. doi: 10.1016/s0304-3835(98)00383-8.