PMID- 10379684
OWN - NLM
STAT- MEDLINE
DCOM- 19990706
LR  - 20190915
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 107
IP  - 6
DP  - 1999 Jun
TI  - An in vitro study of the susceptibility of mobile and cystic forms of Borrelia 
      burgdorferi to metronidazole.
PG  - 566-76
AB  - The aim of this study was to examine the susceptibility of mobile and cystic 
      forms of Borrelia burgdorferi to metronidazole. Because B. burgdorferi is a 
      microaerobic bacterium like Helicobacter pylori, metronidazole (MZ) was chosen in 
      the susceptibility test. For both microaerobic and aerobic incubation the normal 
      mobile spirochetes were resistant to this antibiotic with an MBC > or = 512 
      microg/ml. Conversion of mobile spirochetes to cystic forms was not observed when 
      they were incubated with MZ. When they were incubated under microaerobic 
      conditions, the biologically active cystic forms had an MBC > or = 4 microg/ml, 
      but the MBC was > or = 32 microg/ml with aerobic incubation at 37 degrees C. 
      Staining with acridine orange (AO), dark field microscopy (DFM), and transmission 
      electron microscopy (TEM) revealed that the contents of the cysts were degraded 
      when the concentration of MZ was > or = MBC. Some cysts were also ruptured. When 
      incubated with a sufficient concentration of MZ, core structures did not develop 
      inside the cysts, and AO revealed less RNA in the cysts. Our observations may 
      help efforts to treat resistant infections caused by B. burgdorferi with a 
      combination of MZ and other antibiotics in order to eradicate both cystic and 
      mobile forms of B. burgdorferi.
FAU - Brorson, O
AU  - Brorson O
AD  - Department of Microbiology, Vestfold Sentralsykehus, Tonsberg, Norway.
FAU - Brorson, S H
AU  - Brorson SH
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 140QMO216E (Metronidazole)
SB  - IM
MH  - Borrelia burgdorferi Group/*drug effects/ultrastructure
MH  - Metronidazole/*pharmacology
MH  - Microbial Sensitivity Tests
MH  - Microscopy, Electron
EDAT- 1999/06/24 00:00
MHDA- 1999/06/24 00:01
CRDT- 1999/06/24 00:00
PHST- 1999/06/24 00:00 [pubmed]
PHST- 1999/06/24 00:01 [medline]
PHST- 1999/06/24 00:00 [entrez]
AID - 10.1111/j.1699-0463.1999.tb01594.x [doi]
PST - ppublish
SO  - APMIS. 1999 Jun;107(6):566-76. doi: 10.1111/j.1699-0463.1999.tb01594.x.