PMID- 10381083
OWN - NLM
STAT- MEDLINE
DCOM- 19990810
LR  - 20190831
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 79
IP  - 2
DP  - 1999 May
TI  - A sensitive fluorescence in situ hybridization technique for detection of porcine 
      reproductive and respiratory syndrome virus.
PG  - 133-40
AB  - A sensitive fluorescence in situ hybridization (ISH) for detecting porcine 
      reproductive and respiratory syndrome virus (PRRSV) RNA in viral infected tissue 
      was developed using digoxigenin-labeled RNA probes targeted on the nucleocapsid 
      gene of PRRSV. In situ RNA/RNA hybrids were detected with an anti-digoxigenin 
      antibody alkaline phosphatase conjugate and further revealed with Fast Red TR 
      salt/naphthol AS-MX phosphate using a fluorescent microscope. Viral nucleic acid 
      was readily demonstrated within macrophages, known to be the major target of 
      PRRSV. In addition, positively stained cells were found in the salivary gland and 
      skin tissues which have not been reported to contain PRRSV infected cells before. 
      In conclusion, the fluorescence ISH used in this study provides a fast and 
      sensitive means for screening virus-infected tissues in which relatively few 
      cells are affected. This advantage will be especially beneficial for studying 
      viral persistence and for routine diagnosis of PRRSV infection.
FAU - Chueh, L L
AU  - Chueh LL
AD  - Department of Veterinary Medicine, National Taiwan University, Taipei, ROC. 
      chuehlin@ccms.ntu.edu.tw
FAU - Lee, K H
AU  - Lee KH
FAU - Jeng, C R
AU  - Jeng CR
FAU - Pang, V F
AU  - Pang VF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Coloring Agents)
RN  - 0 (Diazonium Compounds)
RN  - 0 (RNA, Viral)
RN  - 27165-08-8 (Fast Red TA)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Coloring Agents
MH  - Diazonium Compounds
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - RNA, Viral/analysis
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - S016609349900004X [pii]
AID - 10.1016/s0166-0934(99)00004-x [doi]
PST - ppublish
SO  - J Virol Methods. 1999 May;79(2):133-40. doi: 10.1016/s0166-0934(99)00004-x.