PMID- 10381370
OWN - NLM
STAT- MEDLINE
DCOM- 19990729
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 260
IP  - 1
DP  - 1999 Jun 24
TI  - Protease-sensitive urinary pheromones induce region-specific Fos-expression in 
      rat accessory olfactory bulb.
PG  - 222-4
AB  - Vomeronasal organs of female Wistar rats were exposed with sprayed urine 
      preparations of male Wistar rats prior to sacrifice. Exposure to crude urine and 
      ultrafiltrated urine preparation (<5000 Da) induced significant Fos expression, 
      which is correlated with cellular activity, in the mitral/tufted cell layer of 
      the accessory olfactory bulb (AOB), while exposure to the remaining substances 
      after ultrafiltration (>5000 Da) and control salt solution did not. Exposure to 
      urine preparation treated with papain induced expression of Fos-immunoreactive 
      cells in the rostral region of the AOB, but did not induce such expression in the 
      caudal region. Exposure to urine preparation treated with pronase induced 
      urine-specific Fos immunoreactivity neither in the rostral nor in the caudal 
      region. These results suggest that at least two different peptides carrying 
      pheromonal activities are contained in male Wistar rat urine.
CI  - Copyright 1999 Academic Press.
FAU - Tsujikawa, K
AU  - Tsujikawa K
AD  - Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, 
      060-0812, Japan.
FAU - Kashiwayanagi, M
AU  - Kashiwayanagi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Oncogene Proteins v-fos)
RN  - 0 (Pheromones)
RN  - EC 3.4.22.2 (Papain)
RN  - EC 3.4.24.- (Pronase)
SB  - IM
MH  - Animals
MH  - Female
MH  - Male
MH  - Olfactory Bulb/drug effects/*metabolism
MH  - Oncogene Proteins v-fos/analysis/*metabolism
MH  - Papain/pharmacology
MH  - Pheromones/*pharmacology/*urine
MH  - Pronase/pharmacology
MH  - Rats
MH  - Rats, Wistar
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
AID - S0006-291X(99)90873-2 [pii]
AID - 10.1006/bbrc.1999.0873 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jun 24;260(1):222-4. doi: 
      10.1006/bbrc.1999.0873.