PMID- 10381603
OWN - NLM
STAT- MEDLINE
DCOM- 19991020
LR  - 20240317
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 518 ( Pt 2)
IP  - Pt 2
DP  - 1999 Jul 15
TI  - Brain sites of action of endogenous interleukin-1 in the febrile response to 
      localized inflammation in the rat.
PG  - 585-94
AB  - 1. Interleukin (IL)-1 is a potent endogenous pyrogen which causes fever when 
      injected into a number of brain sites. However, the brain sites at which 
      endogenous IL-1 acts to influence body temperature remain equivocal. The aim of 
      this study was to determine the effect of local administration of the 
      interleukin-1 receptor antagonist (IL-1ra) into specific sites in the 
      hypothalamus, and other brain regions known to contain receptors for IL-1, on the 
      febrile response of rats to peripheral injection of lipopolysaccharide (LPS) into 
      a subcutaneous air pouch (intrapouch, i.p.o.) that does not lead to LPS 
      appearance in the circulation. 2. Injection of LPS (100 microgram kg-1, i.p.o.) 
      induced a rise in body temperature which commenced 1.5 h after injection and was 
      maximal at 3 h (38.9 +/- 0.2 C, compared with 37.0 +/- 0.1 C at 0 h, n = 6, P < 
      0.001). Intracerebroventricular (i.c.v.) IL-1ra (500 microgram in 5 microliter) 
      significantly attenuated LPS fever (IL-1ra, 37.7 +/- 0.2 C; saline, 38.9 +/- 0.2 
      C; n = 6, P < 0.001). Unilateral microinjection of IL-1ra (50 microgram in 0.5 
      microliter at 0 + 1 h) into the anterior hypothalamus (AH), paraventricular 
      hypothalamic nucleus (PVH), peri-subfornical organ, subfornical organ (SFO) or 
      hippocampus (dentate gyrus and CA3 region) also significantly reduced the fever 
      induced by LPS. 3. The same dose of IL-1ra had no effect on fever when 
      administered into the ventromedial hypothalamus (VMH), organum vasculosum lamina 
      terminalis (OVLT), CA1 field of the hippocampus, striatum or cortex. 4. These 
      data indicate that the action of endogenous IL-1 in the brain during fever is 
      site specific, acting at the AH, PVH, SFO and hippocampus, but not the VMH, OVLT 
      and striatum or cortex.
FAU - Cartmell, T
AU  - Cartmell T
AD  - School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK.
FAU - Luheshi, G N
AU  - Luheshi GN
FAU - Rothwell, N J
AU  - Rothwell NJ
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Interleukin-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Interleukin-1)
SB  - IM
MH  - Animals
MH  - Body Temperature/drug effects
MH  - Brain/*anatomy & histology
MH  - Brain Chemistry/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Fever/*physiopathology
MH  - Hypothalamus/anatomy & histology/drug effects
MH  - Inflammation/*physiopathology
MH  - Injections, Intraventricular
MH  - Interleukin-1/*physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Microinjections
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Interleukin-1/antagonists & inhibitors
PMC - PMC2269440
EDAT- 1999/06/25 00:00
MHDA- 1999/06/25 00:01
PMCR- 2000/07/15
CRDT- 1999/06/25 00:00
PHST- 1999/06/25 00:00 [pubmed]
PHST- 1999/06/25 00:01 [medline]
PHST- 1999/06/25 00:00 [entrez]
PHST- 2000/07/15 00:00 [pmc-release]
AID - PHY_9044 [pii]
AID - 10.1111/j.1469-7793.1999.0585p.x [doi]
PST - ppublish
SO  - J Physiol. 1999 Jul 15;518 ( Pt 2)(Pt 2):585-94. doi: 
      10.1111/j.1469-7793.1999.0585p.x.