PMID- 10382296 OWN - NLM STAT- MEDLINE DCOM- 19990722 LR - 20161020 IS - 0831-2796 (Print) IS - 0831-2796 (Linking) VI - 42 IP - 3 DP - 1999 Jun TI - Molecular cytogenetic analysis of Aegilops cylindrica host. PG - 497-503 AB - The genomic constitution of Aegilops cylindrica Host (2n = 4x = 28, DcDcCcCc) was analyzed by C-banding, genomic in situ hybridization (GISH), and fluorescence in situ hybridization (FISH) using the DNA clones pSc119, pAs1, pTa71, and pTA794. The C-banding patterns of the Dc- and Cc-genome chromosomes of Ae. cylindrica are similar to those of D-and C-genome chromosomes of the diploid progenitor species Ae. tauschii Coss. and Ae. caudata L., respectively. These similarities permitted the genome allocation and identification of the homoeologous relationships of the Ae. cylindrica chromosomes. FISH analysis detected one major 18S-5.8S-25S rDNA locus in the short arm of chromosome 1Cc. Minor 18S-5.8S-25S rDNA loci were mapped in the short arms of 5Dc and 5Cc. 5S rDNA loci were identified in the short arm of chromosomes 1Cc, 5Dc, 5Cc, and 1Dc. GISH analysis detected intergenomic translocation in three of the five Ae. cylindrica accessions. The breakpoints in all translocations were non-centromeric with similar-sized segment exchanges. FAU - Linc, G AU - Linc G AD - Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvasar, Hungary. FAU - Friebe, B R AU - Friebe BR FAU - Kynast, R G AU - Kynast RG FAU - Molnar-Lang, M AU - Molnar-Lang M FAU - Koszegi, B AU - Koszegi B FAU - Sutka, J AU - Sutka J FAU - Gill, B S AU - Gill BS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PL - Canada TA - Genome JT - Genome JID - 8704544 RN - 0 (DNA, Plant) RN - 0 (DNA, Ribosomal) RN - 0 (RNA, Ribosomal) RN - 0 (RNA, Ribosomal, 18S) RN - 0 (RNA, Ribosomal, 5.8S) RN - 130527-23-0 (RNA, ribosomal, 25S) SB - IM MH - Chromosome Banding MH - *Chromosome Mapping MH - Cloning, Molecular MH - DNA, Plant/genetics MH - DNA, Ribosomal/genetics MH - Edible Grain/*genetics MH - *Genome, Plant MH - In Situ Hybridization MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Plasmids MH - RNA, Ribosomal/genetics MH - RNA, Ribosomal, 18S/genetics MH - RNA, Ribosomal, 5.8S/genetics EDAT- 1999/06/26 00:00 MHDA- 1999/06/26 00:01 CRDT- 1999/06/26 00:00 PHST- 1999/06/26 00:00 [pubmed] PHST- 1999/06/26 00:01 [medline] PHST- 1999/06/26 00:00 [entrez] PST - ppublish SO - Genome. 1999 Jun;42(3):497-503.