PMID- 10386026
OWN - NLM
STAT- MEDLINE
DCOM- 19990720
LR  - 20190822
IS  - 0387-5911 (Print)
IS  - 0387-5911 (Linking)
VI  - 73
IP  - 5
DP  - 1999 May
TI  - [Assay of specific anti-Chlamydia pneumoniae antibodies by ELISA method. 3. 
      Setting of serological criteria].
PG  - 457-66
AB  - "HITAZYME C. pneumoniae" (or "HITAZYME CPN", for short) is a diagnostic reagent 
      that has been recently developed by adopting an ELISA method for detection of 
      anti-Chlamydia pneumoniae (C. pneumoniae) antibodies. When this reagent is used 
      under a current diagnostic standard that has been set as a provisional standard, 
      however, high antibody positive rates are often produced for both IgG and IgA 
      even using the specimens of healthy persons. So, it is difficult to distinguish 
      C. pneumoniae-infected patients from healthy persons. Therefore, this time, we 
      tried to establish a new diagnostic standard by setting up of special cut-off 
      values for a single serum and rise rates of antibody titers for paired sera to 
      improve the accuracy for diagnosis of C. pneumoniae infection. For a single serum 
      testing, we set a special cut-off value at ID 3.00 for both IgG and IgA, so that 
      most healthy persons fall within the range of the "negative" zone. This value was 
      based on the calculation of "Mean+2SD" using measurement results (or IDs) of 
      healthy persons. When this cut-off value was applied, the rate of > or = ID 3.00 
      for either IgG or IgA was 7.6% for healthy persons, and 64.9% for infected 
      patients. (The rate reached 76.4% when the highest IDs of multiple specimens 
      taken from each patient for this test were used in calculation) As a diagnostic 
      standard for a single serum, therefore, it was defined that: "If ID is 3.00 or 
      greater for IgG and/or IgA, it is highly likely that the case has an acute or a 
      present infection." Using paired sera, we could confirm almost a linear 
      relationship between the results by HITAZYME CPN and those by micro-IF method. 
      Under micro-If method, if the antibody titer increases four times or greater 
      using paired sera, acute infection is diagnosed. As it was found that the 
      four-fold increase in antibody titer corresponds to the increase of 1.35 in ID 
      for IgG and 1.00 for IgA, we defined a diagnostic standard for paired sera as 
      follows: "If ID increases by 1.35 or greater for IgG, and/or if ID increases by 
      1.00 or greater for IgA, the case may be diagnosed as acute infection."
FAU - Kishimoto, T
AU  - Kishimoto T
AD  - Department of Health Care Medicine, Kawasaki Medical School.
FAU - Matsushima, T
AU  - Matsushima T
FAU - Morikawa, T
AU  - Morikawa T
FAU - Kawagoe, K
AU  - Kawagoe K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Kansenshogaku Zasshi
JT  - Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious 
      Diseases
JID - 0236671
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Antibodies, Bacterial/*analysis
MH  - Chlamydia Infections/*diagnosis
MH  - Chlamydophila pneumoniae/*immunology
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Reagent Kits, Diagnostic
MH  - Serologic Tests/*standards
EDAT- 1999/07/01 00:00
MHDA- 1999/07/01 00:01
CRDT- 1999/07/01 00:00
PHST- 1999/07/01 00:00 [pubmed]
PHST- 1999/07/01 00:01 [medline]
PHST- 1999/07/01 00:00 [entrez]
AID - 10.11150/kansenshogakuzasshi1970.73.457 [doi]
PST - ppublish
SO  - Kansenshogaku Zasshi. 1999 May;73(5):457-66. doi: 
      10.11150/kansenshogakuzasshi1970.73.457.