PMID- 10386828
OWN - NLM
STAT- MEDLINE
DCOM- 19990824
LR  - 20190921
IS  - 0892-0362 (Print)
IS  - 0892-0362 (Linking)
VI  - 21
IP  - 3
DP  - 1999 May-Jun
TI  - Effect of chronic prenatal ethanol exposure on nitric oxide synthase I and III 
      proteins in the hippocampus of the near-term fetal guinea pig.
PG  - 251-9
AB  - Chronic prenatal ethanol exposure suppresses nitric oxide synthase (NOS) 
      enzymatic activity, in the hippocampus of the near-term fetal guinea pig at 
      gestational day (GD) 62. The objective of this study was to determine if this 
      decrease in NOS activity is the result of decreased NOS I and NOS III protein 
      expression. Pregnant guinea pigs received oral administration of 4 g ethanol/kg 
      maternal body weight/day (n = 8), isocaloric-sucrose/pair feeding (n = 8), or 
      water (n = 8) from GD 2 to GD 61. The NOS I and NOS III protein expression and 
      localization in the hippocampus were determined using Western blot analysis and 
      immunohistochemistry, respectively. The chronic ethanol regimen produced fetal 
      body, brain, and hippocampal growth restriction compared with the 
      isocaloric-sucrose/pair fed and water groups but did not affect the expression or 
      localization of NOS I and NOS III proteins in the hippocampus. The decrease in 
      NOS enzymatic activity induced by chronic prenatal ethanol exposure may be the 
      result of posttranslational modification of NOS I and/or NOS III protein in the 
      hippocampus of the near-term fetal guinea pig.
FAU - Kimura, K A
AU  - Kimura KA
AD  - Department of Pharmacology and Toxicology, Faculty of Health Sciences, Queen's 
      University, Kingston, Ontario, Canada.
FAU - Chiu, J
AU  - Chiu J
FAU - Reynolds, J N
AU  - Reynolds JN
FAU - Brien, J F
AU  - Brien JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neurotoxicol Teratol
JT  - Neurotoxicology and teratology
JID - 8709538
RN  - 3K9958V90M (Ethanol)
RN  - 57-50-1 (Sucrose)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Body Weight/drug effects
MH  - Brain/drug effects/embryology
MH  - Embryonic and Fetal Development
MH  - Ethanol/administration & dosage/blood/*toxicity
MH  - Female
MH  - Fetus/drug effects
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Gestational Age
MH  - Guinea Pigs
MH  - Hippocampus/drug effects/*embryology/*enzymology
MH  - Nitric Oxide Synthase/*genetics/metabolism
MH  - Nitric Oxide Synthase Type I
MH  - Nitric Oxide Synthase Type III
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Protein Processing, Post-Translational
MH  - Sucrose/pharmacology
EDAT- 1999/07/01 00:00
MHDA- 1999/07/01 00:01
CRDT- 1999/07/01 00:00
PHST- 1999/07/01 00:00 [pubmed]
PHST- 1999/07/01 00:01 [medline]
PHST- 1999/07/01 00:00 [entrez]
AID - S0892036298000555 [pii]
AID - 10.1016/s0892-0362(98)00055-5 [doi]
PST - ppublish
SO  - Neurotoxicol Teratol. 1999 May-Jun;21(3):251-9. doi: 
      10.1016/s0892-0362(98)00055-5.