PMID- 10388266
OWN - NLM
STAT- MEDLINE
DCOM- 19990916
LR  - 20190607
IS  - 0023-4001 (Print)
IS  - 1738-0006 (Electronic)
IS  - 0023-4001 (Linking)
VI  - 37
IP  - 2
DP  - 1999 Jun
TI  - Pathogenicity of Korean isolates of Acanthamoeba by observing the experimental 
      infection and zymodemes of five isoenzymes.
PG  - 85-92
AB  - To determine the pathogenicity of Acanthamoeba spp. isolated in Korea and to 
      develop a isoenzymatic maker, the mortality rate of infected mice, in vitro 
      cytotoxicity against target cells and isoenzyme band patterns were observed. Five 
      isolates of Acanthamoeba spp. (YM-2, YM-3, YM-4, YM-5, and YM-7) were used in 
      this study as well as three reference Acanthamoeba spp. (A. culbertsoni, A. 
      hatchetti, and A. royreba). According to the mortality rate of infected mice, 
      Korean isolates could be categorized into three groups high virulent (YM-4), low 
      virulent (YM-2, YM-5, YM-7) and the nonpathogenic group (YM-3). In addition, the 
      virulence of Acanthamoeba spp. was enhanced by brain passage in mice. In the 
      cytotoxicity assay against chinese hamster ovary cells, especially, the 
      cytotoxicity of brain-passaged amoebae was relatively higher than the long-term 
      cultivated ones. The zymodeme patterns of glucose-6-phosphate dehydrogenase 
      (G6PD), malate dehydrogenase (MDH), hexokinase (HK), glutamate oxaloacetate 
      transaminase (GOT) and malic enzyme (ME) of Acanthamoeba spp. were different 
      among each isolate, and also between long-term cultured amoebae and brain 
      passaged ones. In spite of the polymorphic zymodemes, a slow band of G6PD and HK, 
      and an intermediate band of MDH were only observed in pathogenic Acanthamoeba 
      spp., which should be used as isoenzymatic makers.
FAU - Im, K I
AU  - Im KI
AD  - Department of Parasitology, Yonsei University College of Medicine, Seoul, Korea.
FAU - Shin, H J
AU  - Shin HJ
FAU - Seo, D W
AU  - Seo DW
FAU - Jeon, S H
AU  - Jeon SH
FAU - Kim, T E
AU  - Kim TE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - Korean J Parasitol
JT  - The Korean journal of parasitology
JID - 9435800
RN  - 0 (Isoenzymes)
RN  - EC 1.1.1.37 (Malate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Acanthamoeba/enzymology/*pathogenicity
MH  - Amebiasis/*parasitology
MH  - Animals
MH  - Aspartate Aminotransferases/isolation & purification
MH  - CHO Cells
MH  - Cricetinae
MH  - Glucosephosphate Dehydrogenase/isolation & purification
MH  - Hexokinase/isolation & purification
MH  - Isoenzymes/*isolation & purification
MH  - Korea
MH  - Malate Dehydrogenase/isolation & purification
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Virulence
PMC - PMC2733061
EDAT- 1999/07/01 00:00
MHDA- 1999/07/01 00:01
PMCR- 1999/06/01
CRDT- 1999/07/01 00:00
PHST- 1999/07/01 00:00 [pubmed]
PHST- 1999/07/01 00:01 [medline]
PHST- 1999/07/01 00:00 [entrez]
PHST- 1999/06/01 00:00 [pmc-release]
AID - 10.3347/kjp.1999.37.2.85 [doi]
PST - ppublish
SO  - Korean J Parasitol. 1999 Jun;37(2):85-92. doi: 10.3347/kjp.1999.37.2.85.