PMID- 10388650
OWN - NLM
STAT- MEDLINE
DCOM- 19990727
LR  - 20091119
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 259
IP  - 2
DP  - 1999 Jul 5
TI  - The evolution of porcine reproductive and respiratory syndrome virus: 
      quasispecies and emergence of a virus subpopulation during infection of pigs with 
      VR-2332.
PG  - 262-6
AB  - GP5, the principal envelope glycoprotein of porcine reproductive and respiratory 
      syndrome virus (PRRSV), contains a hypervariable region within the ectodomain 
      which is responsible for generating diversity in field isolates. The purpose of 
      this study was to gain insight into the possible origin of this diversity by 
      following GP5 sequence changes in pigs exposed to PRRSV strain VR-2332 in utero. 
      A region of the PRRS virus genome containing portions of ORF4 and ORF5 was 
      amplified directly from tissues of infected pigs from birth to 132 days of age. 
      We observed the emergence of a new PRRSV population, identified by a single 
      nucleotide change in the ectodomain. The Asp to Asn change at amino acid 34 was 
      also found as a minor component in pigs that expressed the wild-type sequence. 
      The results from this study suggest that the variability in the ectodomain of 
      ORF5 is the result of positive or negative selection, of which the mechanism 
      remains to be determined.
CI  - Copyright 1999 Academic Press.
FAU - Rowland, R R
AU  - Rowland RR
AD  - Department of Biology-Microbiology, South Dakota State University, Brookings, 
      South Dakota, 57007, USA. rowlandr@mg.sdstate.edu
FAU - Steffen, M
AU  - Steffen M
FAU - Ackerman, T
AU  - Ackerman T
FAU - Benfield, D A
AU  - Benfield DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - *Genetic Variation
MH  - Lymph Nodes/virology
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Selection, Genetic
MH  - Sequence Analysis, DNA
MH  - Swine
MH  - Umbilical Cord/virology
MH  - Viral Envelope Proteins/chemistry/*genetics/immunology
EDAT- 1999/07/02 00:00
MHDA- 1999/07/02 00:01
CRDT- 1999/07/02 00:00
PHST- 1999/07/02 00:00 [pubmed]
PHST- 1999/07/02 00:01 [medline]
PHST- 1999/07/02 00:00 [entrez]
AID - S0042-6822(99)99789-1 [pii]
AID - 10.1006/viro.1999.9789 [doi]
PST - ppublish
SO  - Virology. 1999 Jul 5;259(2):262-6. doi: 10.1006/viro.1999.9789.