PMID- 10390502
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 14
IP  - 4
DP  - 1999 Jul
TI  - Analysis of bleomycin-induced chromosomal aberrations in Chinese hamster primary 
      embryonic cells by FISH using arm-specific painting probes.
PG  - 357-64
AB  - Chinese hamster primary embryonic cells (at G1 phase) were treated with 1.0 or 
      3.0 microg/ml bleomycin and chromosomal aberrations in first division metaphases 
      were analysed by fluorescence in situ hybridization (FISH) using arm-specific 
      painting probes for chromosomes 3, 4, 8 and 9. We observed that bleomycin induced 
      all classes of chromosome-type aberrations very efficiently. The interesting 
      findings were: (i) the frequency of induced interstitial translocations (i.e. 
      insertions) was approximately equal to that of reciprocal translocations; (ii) 
      the frequency of induced pericentric inversions was higher than that of centric 
      rings. In our earlier studies, we found that X-rays induced a low frequency of 
      interstitial translocations in comparison with reciprocal translocations and 
      equal frequencies of centric rings and pericentric inversions. These data suggest 
      that bleomycin differs from X-rays with respect to the induction of some specific 
      types of aberrations. The results of a chi2 test examining the hypothesis that 
      formed aberrations among the chromosomes or chromosome arms are randomly 
      distributed on the basis of their relative lengths revealed a differential 
      involvement of these chromosomes in the aberrations following exposure to 
      bleomycin. In general, chromosome 8 was found to be more involved in induced 
      aberrations than expected, chromosome 4 was randomly involved, whereas 
      chromosomes 3 and 9 were less involved. This study demonstrates the utility of 
      arm-specific painting probes for efficient detection of a large variety of 
      chromosomal aberrations induced by bleomycin.
FAU - Xiao, Y
AU  - Xiao Y
AD  - MGC Department of Radiation Genetics and Chemical Mutagenesis, Leiden University 
      Medical Centre, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (DNA Probes)
RN  - 11056-06-7 (Bleomycin)
SB  - IM
MH  - Animals
MH  - Antibiotics, Antineoplastic/*toxicity
MH  - Bleomycin/*toxicity
MH  - Cell Line
MH  - *Chromosome Aberrations
MH  - Chromosome Painting/methods
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA Probes
MH  - Embryo, Mammalian
MH  - In Situ Hybridization, Fluorescence
MH  - Male
EDAT- 1999/07/03 00:00
MHDA- 1999/07/03 00:01
CRDT- 1999/07/03 00:00
PHST- 1999/07/03 00:00 [pubmed]
PHST- 1999/07/03 00:01 [medline]
PHST- 1999/07/03 00:00 [entrez]
AID - 10.1093/mutage/14.4.357 [doi]
PST - ppublish
SO  - Mutagenesis. 1999 Jul;14(4):357-64. doi: 10.1093/mutage/14.4.357.