PMID- 10390510
OWN - NLM
STAT- MEDLINE
DCOM- 19990902
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 14
IP  - 4
DP  - 1999 Jul
TI  - The application of comparative genomic hybridization and fluorescence in situ 
      hybridization to the characterization of genotoxicity screening tester strains 
      AHH-1 and MCL-5.
PG  - 417-26
AB  - AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that 
      constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell 
      line was developed by transfection of AHH-1 with cDNAs encoding the human 
      cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase 
      carried in plasmids. The metabolic components of these cell lines make them a 
      useful screening tool for use in mutagenicity studies. Although AHH-1 and MCL-5 
      are closely related, the two cell lines show differences which cannot be 
      attributed to transfection. In the present study both cell lines were 
      investigated for chromosome stability by comparative genomic hybridization (CGH) 
      and fluorescence in situ hybridization (FISH) using whole chromosome probes and 
      telomeric probes. Amplification in chromosomes 4q, 3q and 9p was observed in both 
      cell lines. To compare the cell lines directly, AHH-1 and MCL-5 DNAs were 
      co-hybridized on the same metaphases using a modified CGH technique. The only 
      difference observed between AHH-1 and MCL-5 was the degree of amplification 
      involving the subtelomeric region of chromosome 4; the additional telomeric 
      region (4q) was translocated onto chromosome 11 and/or chromosome X. FISH was use 
      to show the presence of isochromosomes 3q and 9p in both cell lines with a 
      chromosome number of 48 or higher. These data demonstrate that CGH and FISH with 
      chromosome-specific probes are able to resolve complex karyotypes and to 
      highlight subchromosomal regions involved in rearrangements and potential 
      chromosome fragile sites. Analyses such as those described here may be of 
      considerable value in the determination of the stability of a variety of the cell 
      lines used in the mutagenicity testing of chemicals.
FAU - Corso, C
AU  - Corso C
AD  - Center for Molecular Genetics and Toxicology, School of Biological Sciences, 
      University of Wales-Swansea, Swansea SA2 8PP, UK. bacorso@swansea.ac.uk
FAU - Parry, E M
AU  - Parry EM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (DNA Probes)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
SB  - IM
EIN - Mutagenesis 1999 Nov;14(6):658
MH  - Biotransformation
MH  - Cell Line
MH  - Chromosome Aberrations/genetics
MH  - Chromosome Painting/methods
MH  - Cytochrome P-450 Enzyme System/drug effects/metabolism
MH  - DNA Probes
MH  - Humans
MH  - Karyotyping/methods
MH  - Male
MH  - Mutagenicity Tests/*methods
MH  - Nucleic Acid Hybridization/methods
EDAT- 1999/07/03 10:00
MHDA- 2000/03/25 09:00
CRDT- 1999/07/03 10:00
PHST- 1999/07/03 10:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 1999/07/03 10:00 [entrez]
AID - 10.1093/mutage/14.4.417 [doi]
PST - ppublish
SO  - Mutagenesis. 1999 Jul;14(4):417-26. doi: 10.1093/mutage/14.4.417.