PMID- 10391364 OWN - NLM STAT- MEDLINE DCOM- 19991123 LR - 20091119 IS - 0897-7151 (Print) IS - 0897-7151 (Linking) VI - 16 IP - 6 DP - 1999 Jun TI - Expression of brain-derived neurotrophic factor, nerve growth factor, and heat shock protein HSP70 following fluid percussion brain injury in rats. PG - 471-86 AB - Traumatic brain injury can induce the expression of stress-related and neurotrophic genes both within the injury site and in distant regions. These genes may affect severity of damage and/or be neuroprotective. We used in situ hybridization to assess the alterations in expression of the heat shock protein HSP70, nerve growth factor (NGF), and brain-derived neurotrophic factor (BDNF) genes in rat brain following moderate fluid-percussion (F-P) injury at various survival times. HSP70 gene expression was induced at and surrounding the injury site as early as 30 min after trauma. This elevated signal spread ventrally and laterally through the ipsilateral cortex and into the underlying white matter over the next few hours. In addition, there was elevated expression in the temporal hippocampus. BDNF was strongly upregulated in the granular cells of the dentate gyrus and in the CA3 hippocampus 2-6 h after injury. Cortical regions at and near the injury site showed no response at the mRNA level. NGF mRNA increased over the granular cells of the dentate gyrus at early time points. There was also a weaker secondary induction of the NGF gene in the contralateral dentate gyrus of some animals. Cortical response was observed in the entorhinal cortex, bilaterally, but not at the injury site. All three of the studied genes responded quickly to injury, as early as 30 min. The induction of gene expression for neurotrophins in regions remote from areas with histopathology may reflect coupling of gene expression to neuronal excitation, which may be associated with neuroprotection and plasticity. FAU - Truettner, J AU - Truettner J AD - Neurotrauma Research Center, Department of Neurology, University of Miami School of Medicine, Florida 33101, USA. Jessie@stroke.med.miami.edu FAU - Schmidt-Kastner, R AU - Schmidt-Kastner R FAU - Busto, R AU - Busto R FAU - Alonso, O F AU - Alonso OF FAU - Loor, J Y AU - Loor JY FAU - Dietrich, W D AU - Dietrich WD FAU - Ginsberg, M D AU - Ginsberg MD LA - eng GR - NS-05820/NS/NINDS NIH HHS/United States GR - NS-30291/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Neurotrauma JT - Journal of neurotrauma JID - 8811626 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (HSP70 Heat-Shock Proteins) RN - 0 (Nerve Growth Factors) RN - 0 (RNA, Messenger) SB - IM MH - Analysis of Variance MH - Animals MH - Autoradiography MH - Brain Injuries/*metabolism/pathology MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cerebral Cortex/injuries/metabolism/pathology MH - Cortical Spreading Depression/physiology MH - Disease Models, Animal MH - Disease Progression MH - Gene Expression Regulation/*physiology MH - HSP70 Heat-Shock Proteins/*metabolism MH - Hippocampus/injuries/metabolism/pathology MH - In Situ Hybridization MH - Male MH - Nerve Growth Factors/*metabolism MH - Neural Pathways/injuries/metabolism MH - RNA, Messenger/analysis MH - Rats MH - Rats, Sprague-Dawley MH - Time Factors EDAT- 1999/07/03 00:00 MHDA- 1999/07/03 00:01 CRDT- 1999/07/03 00:00 PHST- 1999/07/03 00:00 [pubmed] PHST- 1999/07/03 00:01 [medline] PHST- 1999/07/03 00:00 [entrez] AID - 10.1089/neu.1999.16.471 [doi] PST - ppublish SO - J Neurotrauma. 1999 Jun;16(6):471-86. doi: 10.1089/neu.1999.16.471.