PMID- 10393033
OWN - NLM
STAT- MEDLINE
DCOM- 19990708
LR  - 20111117
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 40
IP  - 8
DP  - 1999 Jul
TI  - Evidence of long-term survival of donor-derived cells after limbal allograft 
      transplantation.
PG  - 1664-8
AB  - PURPOSE: Severe destruction of the corneal limbus causes conjunctival invasion 
      and subsequent visual loss. Limbal allograft transplantation (LAT) was recently 
      proposed for the treatment of these disorders. However, whether the method 
      functions as a stem cell transplantation of the corneal epithelium remains 
      unclear. This study provided evidence that donor-derived corneal epithelial cells 
      survive long after LAT. METHODS: Epithelial cells on the paracentral cornea in 
      patients who have undergone LAT were subjected to fluorescence in situ 
      hybridization (FISH) and polymerase chain reaction restriction fragment length 
      polymorphism (RFLP) analysis. X and Y chromosomes were detected using sex 
      chromosome-specific probes in the FISH analysis, and HLA-DPBI antigens were 
      examined in the RFLP analysis. Eyes receiving conventional penetrating 
      keratoplasty (PKP) served as controls. RESULTS: Donor-derived epithelial cells 
      were detected in three of five eyes (60.0%) in the FISH analysis and in seven of 
      nine eyes (77.8%) in the RFLP analysis. Among these eyes, one and three eyes in 
      the FISH and RFLP analysis, respectively, had both donor- and recipient-derived 
      cells. In control PKP eyes, none of the eyes in the FISH analysis and one of 
      eight eyes (12.5%) in the RFLP analysis had donor-derived cells. CONCLUSIONS: 
      These results suggest that donor-derived cells survive much longer after LAT than 
      those after PKP, and that LAT may function as stem cell transplantation of the 
      corneal epithelium.
FAU - Shimazaki, J
AU  - Shimazaki J
AD  - Department of Ophthalmology, Tokyo Dental College, Chiba, Ichikawa, Japan.
FAU - Kaido, M
AU  - Kaido M
FAU - Shinozaki, N
AU  - Shinozaki N
FAU - Shimmura, S
AU  - Shimmura S
FAU - Munkhbat, B
AU  - Munkhbat B
FAU - Hagihara, M
AU  - Hagihara M
FAU - Tsuji, K
AU  - Tsuji K
FAU - Tsubota, K
AU  - Tsubota K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (HLA-DP Antigens)
RN  - 0 (HLA-DP beta-Chains)
RN  - 0 (HLA-DPw1 antigen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Survival/physiology
MH  - *Cell Transplantation
MH  - Corneal Diseases/*surgery
MH  - DNA/analysis
MH  - Epithelial Cells/transplantation
MH  - Epithelium, Corneal/*cytology/*physiology
MH  - Female
MH  - Follow-Up Studies
MH  - HLA-DP Antigens/analysis
MH  - HLA-DP beta-Chains
MH  - Humans
MH  - Keratoplasty, Penetrating
MH  - Limbus Corneae/*cytology
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Restriction Fragment Length
MH  - Stem Cell Transplantation
MH  - Stem Cells/cytology
MH  - Tissue Donors
MH  - Transplantation, Homologous
MH  - X Chromosome/genetics
MH  - Y Chromosome/genetics
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1664-8.