PMID- 10393322
OWN - NLM
STAT- MEDLINE
DCOM- 19991028
LR  - 20190513
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 126
IP  - 1
DP  - 1999 Jul
TI  - Molecular cloning and functional expression of the human Golgi 
      UDP-N-acetylglucosamine transporter.
PG  - 68-77
AB  - We have cloned the human UDP-N-acetylglucosamine (UDP-GlcNAc) transporter cDNA, 
      which was recognized through a homology search in the expressed sequence tags 
      database (dbEST) based on its similarity to the human UDP-galactose transporter. 
      The chromosomal location of the UDP-GlcNAc transporter gene was assigned to 
      chromosome 1p21 by fluorescence in situ hybridization (FISH). The transporter was 
      expressed ubiquitously in every tissue so far examined. Expression of the 
      transporter cDNA in CHO-K1 cells in its native and in a C-terminally HA-tagged 
      form indicated that the human UDP-GlcNAc transporter was localized in the Golgi 
      apparatus. The membrane vesicles prepared from yeast cells expressing the cDNA 
      product exhibited UDP-GlcNAc-specific transporting activity. Comparison among 
      UDP-galactose, CMP-sialic acid, and UDP-GlcNAc transporters from several 
      organisms enabled us to identify residues highly conserved among the transporters 
      and residues specific for each group of transporters.
FAU - Ishida, N
AU  - Ishida N
AD  - The Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo, 113-8613, 
      Japan.
FAU - Yoshioka, S
AU  - Yoshioka S
FAU - Chiba, Y
AU  - Chiba Y
FAU - Takeuchi, M
AU  - Takeuchi M
FAU - Kawakita, M
AU  - Kawakita M
LA  - eng
SI  - GENBANK/AB021981
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (UDP-N-acetylglucosamine transporter)
RN  - 528-04-1 (Uridine Diphosphate N-Acetylglucosamine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport
MH  - Blotting, Northern
MH  - CHO Cells/metabolism
MH  - Carrier Proteins/*genetics/*metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Golgi Apparatus/*metabolism
MH  - Humans
MH  - *Membrane Transport Proteins
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions
MH  - Uridine Diphosphate N-Acetylglucosamine/*metabolism
EDAT- 1999/07/07 00:00
MHDA- 1999/07/07 00:01
CRDT- 1999/07/07 00:00
PHST- 1999/07/07 00:00 [pubmed]
PHST- 1999/07/07 00:01 [medline]
PHST- 1999/07/07 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022437 [doi]
PST - ppublish
SO  - J Biochem. 1999 Jul;126(1):68-77. doi: 10.1093/oxfordjournals.jbchem.a022437.