PMID- 10394543
OWN - NLM
STAT- MEDLINE
DCOM- 19990819
LR  - 20101118
IS  - 0024-7758 (Print)
IS  - 0024-7758 (Linking)
VI  - 44
IP  - 6
DP  - 1999 Jun
TI  - Advanced FISH with directly labeled X, Y and 18 DNA probes as a tool for rapid 
      prenatal diagnosis.
PG  - 497-503
AB  - OBJECTIVE: To examine a rapid technique for identification and determination of 
      fetal sex chromosomes and one autosome (chromosome 18) in uncultured amniotic 
      fluids using fluorescence in situ hybridization (FISH) with directly labeled DNA 
      probes and ignoring the use of proteinase K and Rnase. STUDY DESIGN: Twenty-five 
      amniotic samples taken from pregnant women who were in their 18th gestational 
      week and had advanced maternal age were studied for analysis of sex chromosomes 
      and chromosome 18 with the FISH technique as well as standard cytogenetic 
      analysis. RESULTS: Four hours after amniocentesis was performed, we identified 
      the sex of the fetuses and disomy of chromosome 18 in a minimal sample of 
      uncultured amniotic fluid by using directly labeled DNA probes for chromosomes X, 
      Y and 18 (VYSIS) and ignoring the use of proteinase K and Rnase. CONCLUSION: The 
      possibility of shortening the time required for identification of aneuploidies in 
      a second-trimester fetus is useful in cases where fetal anomalies are 
      ultrasonically diagnosed at a relatively advanced gestational age.
FAU - Aviram-Goldring, A
AU  - Aviram-Goldring A
AD  - Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Tel 
      Hashomer, Ramat-Gan, Israel.
FAU - Daniely, M
AU  - Daniely M
FAU - Chaki, R
AU  - Chaki R
FAU - Lipitz, S
AU  - Lipitz S
FAU - Barkai, G
AU  - Barkai G
FAU - Goldman, B
AU  - Goldman B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Reprod Med
JT  - The Journal of reproductive medicine
JID - 0173343
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Amniocentesis
MH  - Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 18
MH  - *DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Maternal Age
MH  - Microscopy, Fluorescence
MH  - Pregnancy
MH  - Pregnancy, High-Risk
MH  - *Prenatal Diagnosis
MH  - *Sex Chromosomes
MH  - Sex Determination Processes
MH  - Time Factors
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
PST - ppublish
SO  - J Reprod Med. 1999 Jun;44(6):497-503.