PMID- 10394927
OWN - NLM
STAT- MEDLINE
DCOM- 19990723
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 104
IP  - 5
DP  - 1999 May
TI  - Combined RxFISH/G-banding allows refined karyotyping of solid tumors.
PG  - 370-5
AB  - Chromosome banding analysis of solid tumors often yields incomplete karyotypes 
      because of the complex rearrangements encountered. The addition of fluorescence 
      in situ hybridization (FISH) methods has helped improve the accuracy of solid 
      tumor cytogenetics, but the absence of screening qualities from standard FISH 
      approaches has proved a severe limitation. We describe the cytogenetic analysis 
      of ten solid tumors using G-banding followed by cross-species color banding 
      (RxFISH), a FISH-based screening technique giving a chromosome-specific banding 
      pattern based on the genomic homologies between humans and gibbons. The addition 
      of RxFISH analysis in all cases led to the identification of previously 
      unidentified intra- as well as interchromosomal rearrangements, thus giving a 
      much more certain and detailed karyotype. In two gastric stromal sarcomas, a 
      tumor type for which no cytogenetic data were hitherto available, numerical 
      chromosomal aberrations dominated, but one of the tumors also carried an 
      unbalanced 7;17-translocation with the same breakpoint in chromosome 17 as that 
      seen in endometrial stromal sarcomas.
FAU - Micci, F
AU  - Micci F
AD  - Department of Pathology, The Norwegian Radium Hospital and Institute for Cancer 
      Research, Oslo.
FAU - Teixeira, M R
AU  - Teixeira MR
FAU - Dietrich, C U
AU  - Dietrich CU
FAU - Saeter, G
AU  - Saeter G
FAU - Bjerkehagen, B
AU  - Bjerkehagen B
FAU - Heim, S
AU  - Heim S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/methods
MH  - Chromosome Painting
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping/*methods
MH  - Neoplasms/*genetics/pathology/surgery
MH  - Translocation, Genetic
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
AID - 10.1007/s004390050970 [doi]
PST - ppublish
SO  - Hum Genet. 1999 May;104(5):370-5. doi: 10.1007/s004390050970.