PMID- 10395182
OWN - NLM
STAT- MEDLINE
DCOM- 19990723
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 139
IP  - 2
DP  - 1999 May 24
TI  - Relationship between chromosomal aberrations by fluorescence in situ 
      hybridization and DNA ploidy by cytofluorometry in osteosarcoma.
PG  - 221-6
AB  - An analysis of the chromosomal aberrations and DNA ploidy in the interphase 
      nuclei of seven human osteosacomas was preformed by double-target fluorescence in 
      situ hybridization (FISH) and DNA cytofluorometry. The FISH study of the 
      numerical aberrations in chromosomes 1 and 17 or the structural aberrations in 
      chromosome arm 1p or 17p was carried out by using four locus specific DNA 
      markers, with one pair consisting of 1q12 and 1p36 and the other pair consisting 
      of the 17 cemtromere and 17p13.3. There was no significant differences in the 
      percentage of deletions in chromosome 1 and 17 between osteosarcomas and normal 
      tissues. However, all seven tumors studied had extra copies. Cells with more than 
      three probe signals were regarded as having chromosome polysomy. The percentage 
      of polysomy of chromosome 1 was 20.0-64.0%, and chromosome 17 was 28.0-60.0%. The 
      DNA ploidy patterns of hyperdiploid cells showing a greater DNA content than 
      diploid cells were obtained by DNA cytoflurometry. Five of the seven tumors were 
      non-diploid, and the remaining two were diploid. The percentage of polysomy was 
      correlated with the percentage of hyperdiploid cells in each tumor. Thus, these 
      findings indicated that the DNA ploidy changes were closely correlated with 
      aberrations in the chromosome copy number in osteosarcomas.
FAU - Murata, H
AU  - Murata H
AD  - Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, 
      Japan.
FAU - Kusuzaki, K
AU  - Kusuzaki K
FAU - Hirasawa, Y
AU  - Hirasawa Y
FAU - Ashihara, T
AU  - Ashihara T
FAU - Abe, T
AU  - Abe T
FAU - Inazawa, J
AU  - Inazawa J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Bone Neoplasms/*genetics/secondary
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - DNA, Neoplasm/*genetics
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Osteosarcoma/*genetics/secondary
MH  - *Ploidies
MH  - Tumor Cells, Cultured
EDAT- 1999/07/08 00:00
MHDA- 1999/07/08 00:01
CRDT- 1999/07/08 00:00
PHST- 1999/07/08 00:00 [pubmed]
PHST- 1999/07/08 00:01 [medline]
PHST- 1999/07/08 00:00 [entrez]
AID - S0304383599000427 [pii]
AID - 10.1016/s0304-3835(99)00042-7 [doi]
PST - ppublish
SO  - Cancer Lett. 1999 May 24;139(2):221-6. doi: 10.1016/s0304-3835(99)00042-7.