PMID- 10395541 OWN - NLM STAT- MEDLINE DCOM- 19990917 LR - 20190806 IS - 0960-9822 (Print) IS - 0960-9822 (Linking) VI - 9 IP - 13 DP - 1999 Jul 1 TI - Evidence for functional conservation of a mammalian homologue of the light-responsive plant protein COP1. PG - 711-4 AB - Identified in Arabidopsis as a repressor of light-regulated development, the COP1 (constitutively photomorphogenic 1) protein is characterized by a RING-finger motif and a WD40 repeat domain [1]. The subcellular localization of COP1 is light-dependent. COP1 acts within the nucleus to repress photomorphogenic development, but light inactivates COP1 and diminishes its nuclear abundance [2]. Here, we report the identification of a mammalian COP1 homologue that contains all the structural features present in Arabidopsis COP1 (AtCOP1). When expressed in plant cells, a fusion protein comprising mammalian COP1 and beta-glucuronidase (GUS) responded to light by changing its subcellular localization pattern in a manner similar to AtCOP1. Whereas the mammalian COP1 was unable to rescue the defects of Arabidopsis cop1 mutants, expression of the amino-terminal half of mammalian COP1 in Arabidopsis interfered with endogenous COP1 function, resulting in a hyperphotomorphogenic phenotype. Therefore, the regulatory modules in COP1 proteins that are responsible for the signal-dependent subcellular localization are functionally conserved between higher plants and mammals, suggesting that mammalian COP1 may share a common mode of action with its plant counterpart in regulating development and cellular signaling. FAU - Wang, H AU - Wang H AD - Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104, USA. FAU - Kang, D AU - Kang D FAU - Deng, X W AU - Deng XW FAU - Wei, N AU - Wei N LA - eng SI - GENBANK/AF151110 PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - England TA - Curr Biol JT - Current biology : CB JID - 9107782 RN - 0 (Arabidopsis Proteins) RN - 0 (Carrier Proteins) RN - 0 (Plant Proteins) RN - 0 (Recombinant Fusion Proteins) RN - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis) RN - EC 2.3.2.27 (Ubiquitin-Protein Ligases) RN - EC 3.2.1.31 (Glucuronidase) SB - IM MH - Animals MH - Arabidopsis/*chemistry/growth & development MH - *Arabidopsis Proteins MH - Carrier Proteins/*chemistry/physiology MH - Cells, Cultured MH - Glucuronidase/analysis MH - Humans MH - Light MH - Onions MH - Phenotype MH - Plant Proteins/*chemistry/physiology MH - Recombinant Fusion Proteins/*chemistry/physiology MH - *Ubiquitin-Protein Ligases EDAT- 1999/07/08 00:00 MHDA- 1999/07/08 00:01 CRDT- 1999/07/08 00:00 PHST- 1999/07/08 00:00 [pubmed] PHST- 1999/07/08 00:01 [medline] PHST- 1999/07/08 00:00 [entrez] AID - S0960-9822(99)80314-5 [pii] AID - 10.1016/s0960-9822(99)80314-5 [doi] PST - ppublish SO - Curr Biol. 1999 Jul 1;9(13):711-4. doi: 10.1016/s0960-9822(99)80314-5.