PMID- 10395872 OWN - NLM STAT- MEDLINE DCOM- 19990907 LR - 20091119 IS - 0022-1899 (Print) IS - 0022-1899 (Linking) VI - 180 IP - 2 DP - 1999 Aug TI - Molecular epidemiology of childhood astrovirus infection in child care centers. PG - 514-7 AB - This study assessed the role of human astrovirus (HAstV) in outbreaks and sporadic cases of diarrhea among children attending child care centers (CCCs) and determined the infecting astrovirus antigenic types by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis. Eight astrovirus outbreaks occurred in 6 CCCs. Of 179 children with diarrhea, 36 (20%) had astrovirus-associated diarrhea. Diarrhea stools obtained during diarrhea outbreaks were more likely to contain astrovirus (40/476) than were samples not associated with a diarrhea outbreak (14/452) (P<.001). Type-specific RT-PCR and DNA sequencing identified 5 outbreaks associated with HAstV-1 and 3 outbreaks with HAstV-2. Sequential outbreaks in 2 CCCs occurred with a different type in the same year. Phylogenetic analysis identified 6 clades of HAstV-1 and 2 clades of HAstV-2 during this 1-year surveillance. Astrovirus was a significant cause of diarrhea outbreaks, and 2 antigenic types were present in the community during 1 diarrhea season. FAU - Mitchell, D K AU - Mitchell DK AD - Center for Pediatric Research, Children's Hospital of The King's Daughters, Eastern Virginia Medical School, Norfolk, VA 23510-1001, USA. dkmitchel@chkd.com FAU - Matson, D O AU - Matson DO FAU - Jiang, X AU - Jiang X FAU - Berke, T AU - Berke T FAU - Monroe, S S AU - Monroe SS FAU - Carter, M J AU - Carter MJ FAU - Willcocks, M M AU - Willcocks MM FAU - Pickering, L K AU - Pickering LK LA - eng GR - NICHD-13021/HD/NICHD NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Infect Dis JT - The Journal of infectious diseases JID - 0413675 RN - 0 (DNA, Viral) SB - IM MH - Animals MH - Astroviridae Infections/*epidemiology/*virology MH - *Child Day Care Centers MH - Child, Preschool MH - DNA, Viral/analysis MH - Diarrhea/epidemiology/virology MH - Disease Outbreaks MH - Humans MH - Immunoenzyme Techniques MH - Incidence MH - Infant MH - Mamastrovirus/*classification/genetics/isolation & purification MH - Molecular Epidemiology MH - Phylogeny MH - Reverse Transcriptase Polymerase Chain Reaction EDAT- 1999/07/09 10:00 MHDA- 2000/03/21 09:00 CRDT- 1999/07/09 10:00 PHST- 1999/07/09 10:00 [pubmed] PHST- 2000/03/21 09:00 [medline] PHST- 1999/07/09 10:00 [entrez] AID - JID980845 [pii] AID - 10.1086/314863 [doi] PST - ppublish SO - J Infect Dis. 1999 Aug;180(2):514-7. doi: 10.1086/314863.