PMID- 10398127
OWN - NLM
STAT- MEDLINE
DCOM- 20000330
LR  - 20041117
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 187
IP  - 5
DP  - 1999 Apr
TI  - Further characterization of the human clear cell sarcoma cell line HS-MM 
      demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWS/ATF-1 
      transcript.
PG  - 594-7
AB  - Only a small number of clear cell sarcoma (CCS) cell lines have been reported, 
      including the cell line HS-MM. In the present study, this cell line, maintained 
      for more than 4 years since establishment, was further characterized by 
      cytogenetic studies, fluorescence in situ hybridization (FISH) analysis, and 
      reverse transcriptase-polymerase chain reaction (RT-PCR). HS-MM cells both in 
      vitro and in vivo exhibited pseudodiploid karyotypes with the specific 
      t(12;22)(q13;q12) translocation. The translocation between chromosomes 12 and 22 
      was confirmed by FISH analysis and the hybrid EWS/ATF-1 transcript induced by 
      this translocation was detected by RT-PCR. The HS-MM cell line will be useful for 
      further studies of CCS.
CI  - Copyright 1999 John Wiley & Sons, Ltd.
FAU - Sonobe, H
AU  - Sonobe H
AD  - Department of Pathology, Kochi Medical School, Nankoku, Kochi, Japan.
FAU - Takeuchi, T
AU  - Takeuchi T
FAU - Taguchi, T
AU  - Taguchi T
FAU - Shimizu, K
AU  - Shimizu K
FAU - Iwata, J
AU  - Iwata J
FAU - Furihata, M
AU  - Furihata M
FAU - Ohtsuki, Y
AU  - Ohtsuki Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (EWS-ATF1 fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 22
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoma, Clear Cell/*genetics/pathology
MH  - Transcription Factors/genetics
MH  - *Translocation, Genetic
MH  - *Tumor Cells, Cultured
EDAT- 1999/07/09 10:00
MHDA- 2000/04/01 09:00
CRDT- 1999/07/09 10:00
PHST- 1999/07/09 10:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 1999/07/09 10:00 [entrez]
AID - 10.1002/(SICI)1096-9896(199904)187:5<594::AID-PATH277>3.0.CO;2-H [pii]
AID - 10.1002/(SICI)1096-9896(199904)187:5<594::AID-PATH277>3.0.CO;2-H [doi]
PST - ppublish
SO  - J Pathol. 1999 Apr;187(5):594-7. doi: 
      10.1002/(SICI)1096-9896(199904)187:5<594::AID-PATH277>3.0.CO;2-H.