PMID- 10398426
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 25
IP  - 4
DP  - 1999 Aug
TI  - Cytogenetic clonality analysis of megakaryocytes in myelodysplastic syndrome by 
      dual-color fluorescence in situ hybridization and confocal laser scanning 
      microscopy.
PG  - 332-8
AB  - In the myelodysplastic syndrome (MDS), cytogenetic abnormalities are often 
      present and can be used as markers in studies for cell lineage involvement. 
      Little is known of the involvement of the megakaryocytic lineage due to the 
      variable ploidy of these cells. We applied dual-color fluorescence in situ 
      hybridization (FISH) to routinely prepared bone marrow (BM) smears of 
      cytogenetically normal patients and seven patients with MDS and monosomy 7 or 
      trisomy 8. Probes specific for the centromeric regions of chromosomes 7 and 8 
      were detected with fluorescein isothiocyanate (FITC) and Texas Red, respectively. 
      This enabled us to assess the ratio between the numbers of chromosomes 7 and 8 in 
      the polyploid cells. We utilized confocal laser scanning microscopy to count the 
      FITC and Texas Red FISH signals in the different focal layers of the 
      megakaryocytes. Fifty-six megakaryocytes in six normal BM smears were analyzed, 
      giving a mean ratio of 1.0, a standard deviation (SD) of 0.12, and a range of 
      0.8-1.33. This ratio was applied to evaluation of clonal involvement of 
      individual megakaryocytes in the patients with MDS. In two patients with monosomy 
      7, the majority of the megakaryocytes were monosomic. In the five patients with 
      trisomy 8, all or a majority of the analyzed megakaryocytes were trisomic. These 
      results add direct evidence that in MDS megakaryocytes are involved in the 
      malignant clone. Genes Chromosomes Cancer 25:332-338, 1999.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - van Lom, K
AU  - van Lom K
AD  - Department of Hematology, University Hospital Rotterdam, Dijkzigt, the 
      Netherlands. vanlom@hala.azr.nl
FAU - Houtsmuller, A B
AU  - Houtsmuller AB
FAU - van Putten, W L
AU  - van Putten WL
FAU - Slater, R M
AU  - Slater RM
FAU - Lowenberg, B
AU  - Lowenberg B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Clone Cells
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - Male
MH  - Megakaryocytes/*pathology
MH  - Microscopy, Confocal/methods
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/pathology
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199908)25:4<332::AID-GCC4>3.0.CO;2-4 [pii]
AID - 10.1002/(sici)1098-2264(199908)25:4<332::aid-gcc4>3.3.co;2-w [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1999 Aug;25(4):332-8. doi: 
      10.1002/(sici)1098-2264(199908)25:4<332::aid-gcc4>3.3.co;2-w.