PMID- 10398432
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 25
IP  - 4
DP  - 1999 Aug
TI  - Arylamine N-acetyltransferase type 2 (NAT2), chromosome 8 aneuploidy, and 
      identification of a novel NAT1 cosmid clone: an investigation in bladder cancer 
      by interphase FISH.
PG  - 376-83
AB  - Two genes (arylamine N-acetyltransferase types 1 and 2, NAT1 and NAT2), which are 
      known to metabolize bladder carcinogens, are located on chromosome band 8p22. 
      Alterations in chromosome 8, including deletions of 8p, occur frequently in many 
      epithelium-derived tumors. In this study, fluorescence in situ hybridization 
      (FISH) was used for study of the relationship between chromosome 8 deletions in 
      the region of NAT1 and NAT2 and grade and stage of tumor in bladder cancer. Cells 
      from 52 bladder tumors were examined by dual-labeling FISH with a centromere 
      8-specific probe and a cosmid probe for NAT2. A more limited number were examined 
      for loss with both the NAT2 probe and a newly constructed NAT1-specific cosmid. 
      Loss of NAT2 was found in 6/52 patients in more than 30% of cells, and in 10/52 
      in 10%-30% of cells examined. Six samples also showed loss of NAT1, indicating 
      that the region of deletion spans at least the distance of the two genes. No 
      obvious correlation between loss of NAT genes with grade and stage of tumor was 
      evident. Interestingly, 17/52 (32%) tumors showed an increased copy number of 
      chromosome 8, with tumors of low stage showing relatively smaller increases of 
      chromosome 8. Loss of 8p22 and genetic instability involving chromosome 8 
      indicate that this chromosome is important in bladder cancer and that NAT genes 
      will act as important genetic landmarks in defining deletions in this disease. 
      Genes Chromosomes Cancer 25:376-383, 1999.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Stacey, M
AU  - Stacey M
AD  - Center for Pediatric Research, Eastern Virginia Medical School, Norfolk, 
      Virginia, USA.
FAU - Matas, N
AU  - Matas N
FAU - Drake, M
AU  - Drake M
FAU - Payton, M
AU  - Payton M
FAU - Fakis, G
AU  - Fakis G
FAU - Greenland, J
AU  - Greenland J
FAU - Sim, E
AU  - Sim E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Genetic Markers)
RN  - 0 (Isoenzymes)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.5 (Arylamine N-Acetyltransferase)
RN  - EC 2.3.1.5 (N-acetyltransferase 1)
RN  - EC 2.3.1.5 (NAT2 protein, human)
SB  - IM
MH  - Acetyltransferases/*genetics
MH  - *Aneuploidy
MH  - Arylamine N-Acetyltransferase/*genetics
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Cloning, Molecular
MH  - Cosmids/*genetics
MH  - Genetic Markers
MH  - Genotype
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/genetics
MH  - Isoenzymes
MH  - Neoplasm Staging
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 1999/07/09 00:00
MHDA- 1999/07/09 00:01
CRDT- 1999/07/09 00:00
PHST- 1999/07/09 00:00 [pubmed]
PHST- 1999/07/09 00:01 [medline]
PHST- 1999/07/09 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(199908)25:4<376::AID-GCC10>3.0.CO;2-H [pii]
AID - 10.1002/(sici)1098-2264(199908)25:4<376::aid-gcc10>3.0.co;2-h [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1999 Aug;25(4):376-83. doi: 
      10.1002/(sici)1098-2264(199908)25:4<376::aid-gcc10>3.0.co;2-h.