PMID- 10398931
OWN - NLM
STAT- MEDLINE
DCOM- 19990730
LR  - 20190831
IS  - 0271-3586 (Print)
IS  - 0271-3586 (Linking)
VI  - 36
IP  - 2
DP  - 1999 Aug
TI  - Sperm aneuploidy among Chinese pesticide factory workers: scoring by the FISH 
      method.
PG  - 230-8
AB  - BACKGROUND: A study of the prevalence of sperm aneuploidy among pesticide factory 
      workers was conducted in Anhui, China. METHODS: We recruited 75 men: 32 subjects 
      from a large pesticide-manufacturing plant and 43 subjects from a nearby textile 
      factory free of pesticide exposure. Each subject met the following criteria: age 
      of 20-40 years; continuous work in the plant for 3 months prior to the study, no 
      congenital anomalies or acquired disease of the external genitalia and no history 
      of recent febrile illness or mumps. Within one hour after collection from each 
      subject, semen was evaluated in terms of several parameters and smear slides were 
      prepared. RESULTS: Exposure assessment revealed that workers in the pesticide 
      plant were exposed to ethyl parathion or methamidophos, each of which is a potent 
      organophosphate pesticide, at a median level of 0.02 mg/m3 (8-hour time weighted 
      average as measured by personal pump) while workers in the control plant had no 
      such occupational exposure. Twenty-nine semen slides (13 from the exposed group 
      and 16 from the unexposed group) were randomly chosen for aneuploidy scoring by 
      the three-color fluorescence in situ hybridization (FISH) method with scorers 
      being unaware of exposure status. Median semen parameters were as follows for 
      exposed (and unexposed) men: abstinence period, 3 days (4 days); sperm 
      concentration, 52.8x10(6)/ml (53.1x10(6)/ml); proportion of sperm with normal 
      motility, 50.5% (61.3%); and proportion of sperm with normal morphology, 59% 
      (61.5%). The specific chromosome abnormalities of interest were disomy for 
      chromosome 18 and the three different types of sex chromosome disomy (i.e. XX, 
      XY, YY disomy). The crude proportion of all aneuploidy combined was 0.30% and 
      0.19% for sperm from exposed and unexposed men, respectively. Poisson regression 
      with overdispersion adjustment yielded significantly different crude risks of 
      aneuploidy - 3.03 and 1.94 per 1,000 sperm from exposed and unexposed men, 
      respectively - giving a rate ratio of 1.56 (95% CI, 1.06-2.31). The regression 
      coefficients remained statistically significant after adjustment for 
      inter-technician variability giving a rate ratio of 1.51 (95% CI, 1. 04-2.20). 
      CONCLUSIONS: We conclude that occupational exposure to organophosphate pesticides 
      moderately increases the prevalence of sperm aneuploidy.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Padungtod, C
AU  - Padungtod C
AD  - Occupational Health Program Harvard School of Public Health, Boston, 
      Massachusetts 02115, USA.
FAU - Hassold, T J
AU  - Hassold TJ
FAU - Millie, E
AU  - Millie E
FAU - Ryan, L M
AU  - Ryan LM
FAU - Savitz, D A
AU  - Savitz DA
FAU - Christiani, D C
AU  - Christiani DC
FAU - Xu, X
AU  - Xu X
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Ind Med
JT  - American journal of industrial medicine
JID - 8101110
RN  - 0 (Insecticides)
RN  - 0 (Organothiophosphorus Compounds)
RN  - 41BCL2O91D (Methyl Parathion)
RN  - 61G466064D (Parathion)
RN  - 8Z083FM94W (methamidophos)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Chemical Industry
MH  - China
MH  - Chromosomes, Human, Pair 18/drug effects/genetics
MH  - Confidence Intervals
MH  - DNA/*drug effects
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Insecticides/*adverse effects
MH  - Male
MH  - Methyl Parathion/adverse effects
MH  - Occupational Diseases/*chemically induced/diagnosis
MH  - Occupational Exposure
MH  - Odds Ratio
MH  - Organothiophosphorus Compounds/adverse effects
MH  - Parathion/adverse effects
MH  - Poisson Distribution
MH  - Prevalence
MH  - Regression Analysis
MH  - Sperm Count/drug effects
MH  - Sperm Motility/drug effects
MH  - Spermatozoa/*drug effects
MH  - Textile Industry
MH  - X Chromosome/drug effects/genetics
MH  - Y Chromosome/drug effects/genetics
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
AID - 10.1002/(SICI)1097-0274(199908)36:2<230::AID-AJIM2>3.0.CO;2-6 [pii]
AID - 10.1002/(sici)1097-0274(199908)36:2<230::aid-ajim2>3.0.co;2-6 [doi]
PST - ppublish
SO  - Am J Ind Med. 1999 Aug;36(2):230-8. doi: 
      10.1002/(sici)1097-0274(199908)36:2<230::aid-ajim2>3.0.co;2-6.