PMID- 10399132
OWN - NLM
STAT- MEDLINE
DCOM- 19990909
LR  - 20191103
IS  - 0379-0355 (Print)
IS  - 0379-0355 (Linking)
VI  - 21
IP  - 4
DP  - 1999 May
TI  - Effects of sairei-to and tokishakuyaku-san on cytokine release from peripheral 
      blood mononuclear cells upon recognition of HLA-G protein in the treatment of 
      recurrent abortion.
PG  - 261-4
AB  - Human leukocyte antigen (HLA)-G has been shown to play a role in establishing 
      pregnancy through the mechanism of modulating cytokine secretion from maternal 
      lymphocytes. To elucidate the mechanisms of actions of the herbal medicines, 
      Sairei-to and Tokishakuyaku-san, in the treatment of recurrent abortion, we 
      investigated whether these medicines modulate cytokine secretion from peripheral 
      blood mononuclear cells (PBMCs) upon recognition of HLA-G protein on 
      trophoblasts. Sairei-to and Tokishakuyaku-san increased the interleukin (IL)-1 
      beta and tumor necrosis factor (TNF)-alpha secretion and decreased the IL-3 
      secretion from PBMCs regardless of whether these cells recognized HLA-G protein 
      or not. Accordingly, Sairei-to nullified the effects of HLA-G to reduce the 
      secretion of IL-1 beta and TNF-alpha and to enhance the secretion of IL-3. 
      Tokishakuyaku-san also abolished the effect of HLA-G to reduce IL-1 beta and 
      TNF-alpha secretion but did not affect the increase in IL-3 secretion. Thus, it 
      is conceivable that Sairei-to may normalize Th1/Th2 balance by enhancing Th1 
      polarization in autoimmunity-related recurrent abortion in which Th1/Th2 balance 
      might be shifted towards Th2 polarization. However, the mechanisms of action of 
      Tokishakuyaku-san when used in treating unexplained recurrent abortion, cannot be 
      explained only by the Th1/Th2.
FAU - Fujii, T
AU  - Fujii T
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, University of 
      Tokyo, Japan. fujiit-tky@umin.ac.jp
FAU - Hamai, Y
AU  - Hamai Y
FAU - Kozuma, S
AU  - Kozuma S
FAU - Miki, A
AU  - Miki A
FAU - Yamashita, T
AU  - Yamashita T
FAU - Hyodo, H
AU  - Hyodo H
FAU - Unno, N
AU  - Unno N
FAU - Taketani, Y
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Spain
TA  - Methods Find Exp Clin Pharmacol
JT  - Methods and findings in experimental and clinical pharmacology
JID - 7909595
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Abortion, Habitual/*prevention & control
MH  - Cell Line
MH  - Cytokines/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - HLA Antigens/*immunology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Interleukins/analysis
MH  - Leukocytes, Mononuclear/*drug effects/*immunology
MH  - *Phytotherapy
MH  - Plants, Medicinal/*therapeutic use
MH  - Pregnancy
MH  - Trophoblasts/immunology
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
AID - 538174 [pii]
AID - 10.1358/mf.1999.21.4.538174 [doi]
PST - ppublish
SO  - Methods Find Exp Clin Pharmacol. 1999 May;21(4):261-4. doi: 
      10.1358/mf.1999.21.4.538174.