PMID- 10399964
OWN - NLM
STAT- MEDLINE
DCOM- 19990727
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 82
IP  - 3
DP  - 1999 Jul 30
TI  - Role of fibroblasts in HGF/SF-induced cohort migration of human colorectal 
      carcinoma cells: fibroblasts stimulate migration associated with increased 
      fibronectin production via upregulated TGF-beta1.
PG  - 449-58
AB  - Carcinoma cells frequently invade the surrounding tissue as coherent clusters or 
      nests of cells. We have called this type of movement "cohort migration." We have 
      previously presented an in vitro two-dimensional cohort migration model, in which 
      highly metastatic variant L-10 cells of human rectal adenocarcinoma cell line 
      RCM-1 moved as coherent cell sheets when stimulated with 
      12-O-tetradecanoylphorbol-13-acetate (TPA) or hepatocyte growth factor/scatter 
      factor (HGF/SF). Pericellular deposition of EDA-containing fibronectin (EDA+FN) 
      was essential for TPA-induced cohort migration. In this study, we investigated 
      how colon-derived fibroblasts could affect the induction of cohort migration of 
      colorectal carcinoma cells by HGF/SF, since carcinoma cell-fibroblast 
      interactions frequently regulate biological events during cancer cell invasion. 
      Fibroblasts co-cultured with L-10 carcinoma cells stimulated HGF/SF-induced 
      cohort migration of L-10 cells up to 2 to 3-fold. Conditioned medium (CM) from 
      fibroblasts that were cultured alone was not effective but CM from fibroblasts 
      cocultured with carcinoma cells enhanced HGF/SF-induced cohort migration, and 
      this effect in CM was found to be mediated by TGF-beta1 upregulated in 
      co-cultured conditions. Among the motogenic growth factors examined, only 
      TGF-beta1 synergistically stimulated HGF/SF-induced L-10 cell cohort migration, 
      although TGF-beta1 alone did not induce cohort migration. TGF-beta1 also 
      exhibited synergistic effect in several other human colorectal carcinoma cell 
      lines. The synergistic stimulation of L-10 cell cohort migration by HGF/SF and 
      TGF-beta1 was associated with increased production of motility-enhancing EDA+FN 
      by L-10 cells, and blocking FN with a specific antibody effectively inhibited the 
      synergistic effect.
FAU - Shimao, Y
AU  - Shimao Y
AD  - Department of Pathology, Miyazaki Medical College, Kiyotake, Japan.
FAU - Nabeshima, K
AU  - Nabeshima K
FAU - Inoue, T
AU  - Inoue T
FAU - Koono, M
AU  - Koono M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Fibronectins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Adenocarcinoma/*pathology
MH  - Cell Movement/drug effects
MH  - Colorectal Neoplasms/*pathology
MH  - Drug Synergism
MH  - Fibroblasts/drug effects
MH  - Fibronectins/*biosynthesis
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - Recombinant Proteins/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stimulation, Chemical
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Tumor Cells, Cultured
MH  - Up-Regulation
EDAT- 1999/07/10 10:00
MHDA- 2000/06/20 09:00
CRDT- 1999/07/10 10:00
PHST- 1999/07/10 10:00 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/07/10 10:00 [entrez]
AID - 10.1002/(SICI)1097-0215(19990730)82:3<449::AID-IJC20>3.0.CO;2-H [pii]
AID - 10.1002/(sici)1097-0215(19990730)82:3<449::aid-ijc20>3.0.co;2-h [doi]
PST - ppublish
SO  - Int J Cancer. 1999 Jul 30;82(3):449-58. doi: 
      10.1002/(sici)1097-0215(19990730)82:3<449::aid-ijc20>3.0.co;2-h.