PMID- 10400044
OWN - NLM
STAT- MEDLINE
DCOM- 19990805
LR  - 20190514
IS  - 0003-4932 (Print)
IS  - 1528-1140 (Electronic)
IS  - 0003-4932 (Linking)
VI  - 230
IP  - 1
DP  - 1999 Jul
TI  - Acceptance of skin allografts in pigs by portal venous injection of donor bone 
      marrow cells.
PG  - 114-9
AB  - OBJECTIVE: To confirm in pigs whether a new method for organ allografts, 
      originally established in mice by the authors, might be applicable to humans. 
      SUMMARY BACKGROUND DATA: The authors recently established a new method for organ 
      allografts in mice that includes the injection of donor bone marrow cells (BMCs) 
      using the portal vein (PV), followed by the administration of cyclosporin A (CsA) 
      on days 2 and 5, and the intravenous injection of BMCs on day 5. In the present 
      study, they modify this method (a single-day protocol) and apply it to pigs. 
      METHODS: Allogeneic BMCs of donor pigs were injected using the PV (a superior 
      mesenteric vein). The skin grafting was carried out on the day of the PV 
      injection. The recipient pigs received donor grafts, autologous grafts, and 
      third-party grafts at the same time. In addition, an open wound was made as the 
      epithelized control. Full-thickness skin grafts were harvested from the dorsal 
      wall of the donors. CsA (10 mg/kg) was injected intramuscularly into recipient 
      pigs on days 2 and 5 after the PV injection. RESULTS: One hundred percent of skin 
      grafts survived for >300 days when donor BMCs were injected using the PV (n = 6). 
      However, the skin grafts of the three pigs that had received BMCs using the 
      intravenous route were rejected within 3 to 4 weeks after transplantation. The 
      third-party skin grafts showed necrotic changes on day 21 after transplantation. 
      CONCLUSIONS: One hundred percent of skin allografts can be obtained, even in 
      pigs, by injecting donor BMCs using the PV, carrying out skin allografts, and 
      administering CsA on days 2 and 5. This single-day protocol would be of great 
      advantage for human organ transplantation.
FAU - Morita, H
AU  - Morita H
AD  - First Department of Surgery, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Nakamura, N
AU  - Nakamura N
FAU - Sugiura, K
AU  - Sugiura K
FAU - Satoi, S
AU  - Satoi S
FAU - Sakakura, Y
AU  - Sakakura Y
FAU - Tu, W
AU  - Tu W
FAU - Yoshida, K
AU  - Yoshida K
FAU - Oda, M
AU  - Oda M
FAU - Inoue, T
AU  - Inoue T
FAU - Inui, H
AU  - Inui H
FAU - Nagahama, T
AU  - Nagahama T
FAU - Kamiyama, Y
AU  - Kamiyama Y
FAU - Ikehara, S
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Surg
JT  - Annals of surgery
JID - 0372354
SB  - IM
MH  - Animals
MH  - *Bone Marrow Cells
MH  - *Graft Survival
MH  - Injections
MH  - Male
MH  - Portal Vein
MH  - Skin Transplantation/*immunology
MH  - Swine
MH  - *Tissue Donors
PMC - PMC1420852
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
PMCR- 2000/01/01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
PHST- 2000/01/01 00:00 [pmc-release]
AID - 0000658-199907000-00016 [pii]
AID - 10.1097/00000658-199907000-00016 [doi]
PST - ppublish
SO  - Ann Surg. 1999 Jul;230(1):114-9. doi: 10.1097/00000658-199907000-00016.