PMID- 10400107
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20190817
IS  - 0277-2116 (Print)
IS  - 0277-2116 (Linking)
VI  - 29
IP  - 1
DP  - 1999 Jul
TI  - Amelioration of ischemia-reperfusion injury in rat intestine by 
      pentoxifylline-mediated inhibition of xanthine oxidase.
PG  - 69-74
AB  - BACKGROUND: Intestinal ischemia-reperfusion (IR) injury results in cell 
      destruction, which may be mediated by the generation of reactive oxygen species, 
      potentially toxic metabolites of xanthine oxidase. Pentoxifylline (PTX) possesses 
      a variety of biochemical and antioxidant properties that can improve capillary 
      flow and tissue oxygenation. Because of these combined effects, it has been 
      hypothesized that pentoxifylline would protect against intestinal IR. METHODS: 
      Young adult rats were randomly assigned to one of four experimental groups: 
      IR/Placebo (n = 12) in which superior and inferior mesenteric arteries were 
      clamped for 45 minutes and then reopened; IR/PTX (n = 11) in which IR was induced 
      as in the Placebo group, but with 25 mg/kg PTX at 0, 30, and 60 minutes; No 
      IR/Placebo (n = 12); and No IR/PTX (n = 6) in which placebo and PTX were applied 
      with no IR. Blood and intestinal samples were taken for serial thiobarbituric 
      acid-reducing substances (TBARS; index of lipid peroxidation), for xanthine 
      oxidase-xanthine dehydrogenase ratios, glutathione, myeloperoxidase, and 
      histopathology. RESULTS: Animals in the IR/PTX group had lower TBARS and the 
      least severe histopathologic injury. Xanthine oxidasexanthine dehydrogenase 
      ratios were elevated only in IR/ Placebo (0.67+/-0.22 vs. 0.45+/-0.14 in IR/PTX; 
      0.42+/-0.22 in No IR/Placebo; and 0.40+/-0.11 in No IR/PTX; p = 0.0009). Reduced 
      glutathione was diminished in IR/PTX animals (38.9 +/-1.35 vs. 46.1+/-7.0 in 
      IR/Placebo; 41.1+/-2.5 in No IR/ Placebo; 43.6+/-1.0 in No IR/PTX; p = 0.048). No 
      differences were recorded in myeloperoxidase levels among groups. CONCLUSIONS: 
      Pentoxifylline ameliorates histopathologic signs of injury and decreases lipid 
      peroxidation (TBARS). Normal xanthine oxidase-xanthine dehydrogenase ratios in 
      the treated compared with IR-only animals imply that the protective effect of PTX 
      is at least partially mediated through inhibition of xanthine oxidase.
FAU - Hammerman, C
AU  - Hammerman C
AD  - Shaare Zedek Medical Center and the Hebrew University-Hadassah Medical School, 
      Jerusalem, Israel.
FAU - Goldschmidt, D
AU  - Goldschmidt D
FAU - Caplan, M S
AU  - Caplan MS
FAU - Kaplan, M
AU  - Kaplan M
FAU - Schimmel, M S
AU  - Schimmel MS
FAU - Eidelman, A I
AU  - Eidelman AI
FAU - Branski, D
AU  - Branski D
FAU - Hochman, A
AU  - Hochman A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Gastroenterol Nutr
JT  - Journal of pediatric gastroenterology and nutrition
JID - 8211545
RN  - 0 (Free Radical Scavengers)
RN  - EC 1.17.3.2 (Xanthine Oxidase)
RN  - SD6QCT3TSU (Pentoxifylline)
SB  - IM
MH  - Animals
MH  - Evaluation Studies as Topic
MH  - Free Radical Scavengers/*pharmacology
MH  - Intestines/*blood supply/pathology
MH  - Lipid Peroxidation
MH  - Pentoxifylline/*pharmacology
MH  - Random Allocation
MH  - Rats
MH  - Reperfusion Injury/pathology/physiopathology/*prevention & control
MH  - Xanthine Oxidase/*antagonists & inhibitors
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
AID - 10.1097/00005176-199907000-00017 [doi]
PST - ppublish
SO  - J Pediatr Gastroenterol Nutr. 1999 Jul;29(1):69-74. doi: 
      10.1097/00005176-199907000-00017.