PMID- 10400183
OWN - NLM
STAT- MEDLINE
DCOM- 19990716
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 23
IP  - 7
DP  - 1999 Jul
TI  - Quantification of human herpesvirus 6 in healthy volunteers and patients with 
      lymphoproliferative disorders by PCR-ELISA.
PG  - 625-30
AB  - To determine whether actual numbers of human herpesvirus 6 (HHV-6) genome in 
      hematologic neoplasias are associated with disease condition, we developed a 
      quantitative PCR-ELISA for detection of HHV-6. The amount of viral DNA was 
      determined using externally amplified known amounts of the plasmid DNA containing 
      the viral target sequences. First, we determined a viral burden in peripheral 
      blood leukocytes obtained from 23 healthy volunteers and four specimens of lymph 
      nodes with reactive hyperplasia. Using 1 microg of DNA, the prevalence of HHV-6 
      was 43.4% (10/23), ranging from 0 to 100 HHV-6 genomes in blood obtained from 
      healthy volunteers. The amounts of HHV-6 genomes were < 10 in four non-neoplastic 
      lymph node specimens. We next examined the amount of viral DNA in 21 blood 
      specimens and 19 lymph node specimens obtained from patients with 
      lymphoproliferative diseases (LPD) at the time of diagnosis. The number of HHV-6 
      genomes in most of the B-cell lymphoma was < 5 in both blood and lymph node 
      specimens, however, two lymph node specimens obtained from immunoblastic 
      lymphadenopathy (IBL) and T-cell lymphoma had very high levels of HHV-6 viral DNA 
      (3705 and 810, respectively). We also found that HHV-6 genomes in peripheral 
      blood were more than 1000 in two patients with chronic lymphocytic leukemia. For 
      all LPD patients combined, there were significantly higher levels of viral DNA 
      (200.6 +/- 654.8 HHV-6 genomes per 1 microg purified DNA) compared to those in 
      healthy volunteers (10.0 +/- 21.0 HHV-6 genomes per 1 microg purified DNA) (P < 
      0.05). This study demonstrates that a high level of HHV-6 viral DNA is 
      occasionally associated with LPD patients. Although it is still uncertain whether 
      HHV-6 is related to the pathogenesis in LPD or not, our results suggest that 
      measurement of HHV-6 genomes using PCR-ELISA may be useful not only to understand 
      the mechanism of HHV-6 infection in hemopoietic neoplasia but also to manage the 
      care of immnocompromised patients such as bone marrow transplant patients.
FAU - Ohyashiki, J H
AU  - Ohyashiki JH
AD  - Department of Virology, Medical Research Institute, Tokyo Medical and Dental 
      University, Japan. junko@hh.iij4u.or.jp
FAU - Abe, K
AU  - Abe K
FAU - Ojima, T
AU  - Ojima T
FAU - Wang, P
AU  - Wang P
FAU - Zhou, C F
AU  - Zhou CF
FAU - Suzuki, A
AU  - Suzuki A
FAU - Ohyashiki, K
AU  - Ohyashiki K
FAU - Yamamoto, K
AU  - Yamamoto K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - DNA, Neoplasm/genetics
MH  - DNA, Viral/*isolation & purification
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Genome, Viral
MH  - Herpesviridae Infections/complications/epidemiology/*virology
MH  - Herpesvirus 6, Human/classification/genetics/*isolation & 
      purification/pathogenicity
MH  - Humans
MH  - Leukocytes, Mononuclear/virology
MH  - Lymph Nodes/virology
MH  - Lymphoproliferative Disorders/complications/*virology
MH  - Male
MH  - Middle Aged
MH  - Neoplastic Stem Cells/virology
MH  - Polymerase Chain Reaction
MH  - Prevalence
EDAT- 1999/07/10 00:00
MHDA- 1999/07/10 00:01
CRDT- 1999/07/10 00:00
PHST- 1999/07/10 00:00 [pubmed]
PHST- 1999/07/10 00:01 [medline]
PHST- 1999/07/10 00:00 [entrez]
AID - S014521269900079X [pii]
AID - 10.1016/s0145-2126(99)00079-x [doi]
PST - ppublish
SO  - Leuk Res. 1999 Jul;23(7):625-30. doi: 10.1016/s0145-2126(99)00079-x.