PMID- 10401528
OWN - NLM
STAT- MEDLINE
DCOM- 19990924
LR  - 20190717
IS  - 0003-9969 (Print)
IS  - 0003-9969 (Linking)
VI  - 44
IP  - 6
DP  - 1999 Jun
TI  - Distribution of interleukin-2, -4, -10, tumour necrosis factor-alpha and 
      transforming growth factor-beta mRNAs in oral lichen planus.
PG  - 499-507
AB  - In the present study, MRNA for the cytokines interleukin-2 (IL-2), IL-4, IL-10 
      tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor beta-1 
      (TGF-beta-1) were investigated in oral lichen planus (OLP) lesions using in situ 
      hybridization with 35S-labelled oligonucleotide probes on frozen tissue sections. 
      In addition, the expression of interferon-gamma (IFN-gamma), IL-10 and IL-4 mRNAs 
      was analysed in cultured lesional T lymphocytes from oral lichen planus by 
      polymerase chain reaction. Cells expressing mRNA for IL-2, IL-4, IL-10, TNF-alpha 
      and TGF-beta 1 were found in all the biopsies studied. Approximately 1-2% of the 
      total number of infiltrating cells in the lesions were positive for each of the 
      different cytokine mRNAs. Most biopsies contained basement membrane-oriented, 
      mRNA-positive cells. In the cultured T-cell lines, message for IFN-gamma was 
      detected in all the patients, IL-10 in all but one, and IL-4 in just one of the 
      seven patients investigated. The results suggest that mRNA for both pro- and 
      anti-inflammatory cytokines, i.e., mixed T-helper 1 (TH1) and TH2 cytokine 
      profiles, are generated simultaneously by a limited number of cells in chronic 
      lesions of OLP.
FAU - Simark-Mattsson, C
AU  - Simark-Mattsson C
AD  - Department of Endodontology/Oral Diagnosis, Goteborg University, Sweden. 
      charlotte.simark@odontolgi.gu.se
FAU - Bergenholtz, G
AU  - Bergenholtz G
FAU - Jontell, M
AU  - Jontell M
FAU - Eklund, C
AU  - Eklund C
FAU - Seymour, G J
AU  - Seymour GJ
FAU - Sugerman, P B
AU  - Sugerman PB
FAU - Savage, N W
AU  - Savage NW
FAU - Dahlgren, U I
AU  - Dahlgren UI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Interleukin-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cells, Cultured
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Interleukin-10/*metabolism
MH  - Interleukin-2/*metabolism
MH  - Interleukin-4/*metabolism
MH  - Lichen Planus, Oral/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - T-Lymphocytes/metabolism
MH  - Transforming Growth Factor beta/*metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1999/07/13 00:00
MHDA- 1999/07/13 00:01
CRDT- 1999/07/13 00:00
PHST- 1999/07/13 00:00 [pubmed]
PHST- 1999/07/13 00:01 [medline]
PHST- 1999/07/13 00:00 [entrez]
AID - S0003-9969(99)00013-8 [pii]
AID - 10.1016/s0003-9969(99)00013-8 [doi]
PST - ppublish
SO  - Arch Oral Biol. 1999 Jun;44(6):499-507. doi: 10.1016/s0003-9969(99)00013-8.