PMID- 10402634
OWN - NLM
STAT- MEDLINE
DCOM- 19990824
LR  - 20220316
IS  - 8756-0437 (Print)
IS  - 1098-2388 (Linking)
VI  - 17
IP  - 1
DP  - 1999 Jul-Aug
TI  - New diagnostic modalities in soft tissue sarcoma.
PG  - 11-22
AB  - Molecular and cytogenetic analysis of soft tissue sarcoma has provided a vast 
      amount of new genetic information over the past 10 years. Recent advances in 
      genetic technology, such as fluorescence in situ hybridization (FISH), reverse 
      transcriptase-polymerase chain reaction (RT-PCR), and positional cloning 
      techniques have greatly increased the rate of new discoveries and soon may bring 
      cytogenetic and molecular analysis to standard pathology laboratories. Karotypic 
      analysis of soft tissue tumors have demonstrated specific cytogenetic aberrations 
      which have proved to be extremely useful diagnostically and have solidified and 
      improved soft tissue tumor classification systems. Objective and reproducible 
      prognostication in soft tissue sarcoma remains problematic. Presently, the grade 
      and size of the sarcoma are the most important factors used to estimate risk of 
      relapse and overall survival. Assigning a pathologic grade to an individual 
      sarcoma as a means of predicting clinical behavior is often difficult with a 40% 
      discordance rate even between expert sarcoma pathologists. There is mounting 
      evidence that the composition of membrane phospholipid in tumor tissue is an 
      important indicator of a tumor's cellularity, proliferative capacity, and 
      differentiation state. However, there is a lack of information on the biochemical 
      determinants of sarcoma proliferation and differentiation. To address these 
      problems, novel quantitative ex vivo nuclear magnetic resonance (NMR) methods 
      have been applied to determine the biochemical changes in tissue lipid for soft 
      tissue sarcoma. The biochemical changes in tissue lipid have been found to 
      correlate with sarcoma cellularity, growth rate, and differentiation. Continued 
      prospective NMR analysis of tissue lipid biochemistry in soft tissue tumors will 
      permit the development of a clinically relevant biochemical system of prognostic 
      determinants for soft tissue sarcoma in the future.
FAU - Singer, S
AU  - Singer S
AD  - Harvard Medical School, Boston, Massachussetts, USA.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - United States
TA  - Semin Surg Oncol
JT  - Seminars in surgical oncology
JID - 8503713
SB  - IM
MH  - Cytogenetics
MH  - Genetic Techniques
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Biology
MH  - Prognosis
MH  - Sarcoma/chemistry/*diagnosis/genetics
MH  - Soft Tissue Neoplasms/chemistry/*diagnosis/genetics
RF  - 78
EDAT- 1999/07/14 10:00
MHDA- 2000/06/22 10:00
CRDT- 1999/07/14 10:00
PHST- 1999/07/14 10:00 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1999/07/14 10:00 [entrez]
AID - 10.1002/(SICI)1098-2388(199907/08)17:1<11::AID-SSU3>3.0.CO;2-0 [pii]
AID - 10.1002/(sici)1098-2388(199907/08)17:1<11::aid-ssu3>3.0.co;2-0 [doi]
PST - ppublish
SO  - Semin Surg Oncol. 1999 Jul-Aug;17(1):11-22. doi: 
      10.1002/(sici)1098-2388(199907/08)17:1<11::aid-ssu3>3.0.co;2-0.