PMID- 10403426
OWN - NLM
STAT- MEDLINE
DCOM- 19991012
LR  - 20190831
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 35
IP  - 1
DP  - 1999 Jul
TI  - Increased expression of glyceraldehyde-3-phosphate dehydrogenase in cultured 
      astrocytes following exposure to manganese.
PG  - 11-7
AB  - Manganism is a disorder characterized by hyperintensities in basal ganglia 
      structures on magnetic resonance imaging which may be the consequence of 
      manganese deposition in these areas. Since manganese is taken up avidly into 
      astrocytes and is known to interfere with cerebral energy metabolism, we studied 
      the effect of this metal on the expression and activity of the glycolytic enzyme 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in primary cultures of 
      astrocytes. Treatment with 100 microM manganese for 7 days increased both the 
      Vmax and Km values for GAPDH which was not reproducible with other divalent 
      metals. Using RT-PCR, increased GAPDH expression was detected in cells exposed to 
      manganese compared with controls. No changes in cytochrome oxidase activity or 
      ATP levels were observed, and lactate production was unaffected, in 
      manganese-treated cells. These findings provide evidence of a possible role for 
      GAPDH in the mediation of the effects of manganese on central nervous system 
      function.
FAU - Hazell, A S
AU  - Hazell AS
AD  - Neuroscience Research Unit, Hopital Saint-Luc (University of Montreal), Quebec, 
      Canada.
FAU - Desjardins, P
AU  - Desjardins P
FAU - Butterworth, R F
AU  - Butterworth RF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Chlorides)
RN  - 0 (Manganese Compounds)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - QQE170PANO (manganese chloride)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/cytology/*enzymology
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chlorides/*pharmacology
MH  - Electron Transport Complex IV/metabolism
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*genetics/*metabolism
MH  - Kinetics
MH  - L-Lactate Dehydrogenase/analysis
MH  - Manganese Compounds/*pharmacology
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/07/14 00:00
MHDA- 1999/07/14 00:01
CRDT- 1999/07/14 00:00
PHST- 1999/07/14 00:00 [pubmed]
PHST- 1999/07/14 00:01 [medline]
PHST- 1999/07/14 00:00 [entrez]
AID - S0197-0186(99)00024-8 [pii]
AID - 10.1016/s0197-0186(99)00024-8 [doi]
PST - ppublish
SO  - Neurochem Int. 1999 Jul;35(1):11-7. doi: 10.1016/s0197-0186(99)00024-8.