PMID- 10404434
OWN - NLM
STAT- MEDLINE
DCOM- 19990928
LR  - 20191103
IS  - 1524-9557 (Print)
IS  - 1524-9557 (Linking)
VI  - 22
IP  - 4
DP  - 1999 Jul
TI  - Neuroblastoma cells expressing mature IL-18, but not proIL-18, induce a strong 
      and immediate antitumor immune response.
PG  - 324-35
AB  - Retroviral constructs were designed to express the novel cytokine interleukin 18 
      (IL-18), also known as interferon-gamma-inducing factor, in a murine 
      neuroblastoma cell line [neuro-2a (N-2a)] to examine the effects of IL-18 
      expression on tumorigenicity. N-2a cells expressing proIL-18 (N-2a/IL-18p) were 
      as tumorigenic as parental N-2a cells, whereas N-2a cells engineered to secrete 
      mature IL-18 (N-2a/IL-18m) were nontumorigenic. Inoculation of mice with 
      N-2a/IL-18m generated immediate immunity to parental N-2a. N-2a/IL-18m formed 
      tumors in mice depleted of CD4+ and CD8+ T cells, suggesting that the antitumor 
      immune response was T cell mediated. The resulting T-helper (Th) immune response 
      was also characterized in vitro and had a large Th1 component based on in vitro 
      production of the cytokines IFN-gamma and granulocyte macrophage 
      colony-stimulating factor in response to tumor cells and IL-18.
FAU - Heuer, J G
AU  - Heuer JG
AD  - Herman B Wells Center for Pediatric Research, Department of Pediatrics, Indiana 
      University School of Medicine, Indianapolis, USA.
FAU - Tucker-McClung, C
AU  - Tucker-McClung C
FAU - Hock, R A
AU  - Hock RA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Il18r1 protein, mouse)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Protein Precursors)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
RN  - 0 (interleukin 1 precursor)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Base Sequence
MH  - Cytokines/analysis/biosynthesis/*immunology
MH  - Female
MH  - Genetic Vectors/biosynthesis
MH  - Immunocompromised Host/*immunology
MH  - Immunotherapy/methods
MH  - Interleukin-1/biosynthesis/*immunology
MH  - Interleukin-18/biosynthesis/*immunology
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Mice
MH  - Mice, Inbred A
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Neuroblastoma/*immunology
MH  - Polymerase Chain Reaction
MH  - Protein Precursors/biosynthesis/*immunology
MH  - Radioimmunoassay
MH  - Receptors, Interleukin/antagonists & inhibitors/genetics
MH  - Receptors, Interleukin-18
MH  - Reference Values
MH  - Retroviridae/genetics
MH  - Spleen/immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology
MH  - Transduction, Genetic
MH  - Tumor Cells, Cultured/immunology/metabolism
EDAT- 1999/07/15 00:00
MHDA- 1999/07/15 00:01
CRDT- 1999/07/15 00:00
PHST- 1999/07/15 00:00 [pubmed]
PHST- 1999/07/15 00:01 [medline]
PHST- 1999/07/15 00:00 [entrez]
AID - 10.1097/00002371-199907000-00006 [doi]
PST - ppublish
SO  - J Immunother. 1999 Jul;22(4):324-35. doi: 10.1097/00002371-199907000-00006.