PMID- 10405340
OWN - NLM
STAT- MEDLINE
DCOM- 19990809
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 261
IP  - 1
DP  - 1999 Jul 22
TI  - Identification and localization of MEP1A-like sequences (MEP1AL1-4) in the human 
      genome.
PG  - 163-8
AB  - The human MEP1A gene encodes the meprin alpha subunit that consists of a protease 
      domain conserved in the astacin family of metalloendopeptidases and several 
      C-terminal interaction domains present in other proteins. Using the alpha subunit 
      cDNA, we identified two clones from a human P1-derived artificial chromosome 
      (PAC) library. Fluorescence in situ hybridization (FISH) mapped both PACs (1e12, 
      65a14) to chromosome 6p21, confirming the MEP1A location. FISH also mapped PAC 
      65a14 to chromosome 13cen, and to chromosome 9 in three different regions, 
      9p12-13, 9q21, and 9q22. Southern blot analysis showed that sequences of PAC 
      65a14 and MEP1A were similar in the 3' end but different in the 5' end, revealing 
      for the first time that the human genome may encode multiple interaction domains 
      highly similar to those of the meprin alpha subunit. The symbols of MEP1AL1, 
      MEP1AL2, MEP1AL3, and MEP1AL4 have been designated for MEP1A-like sequences on 
      9p12-13, 9q21, 9q22, and 13cen, respectively.
CI  - Copyright 1999 Academic Press.
FAU - Jiang, W
AU  - Jiang W
AD  - Department of Biochemistry and Molecular Biology, Pennsylvania State University 
      College of Medicine, Hershey, Pennsylvania, 17033, USA. wjiang@psu.edu
FAU - Beatty, B G
AU  - Beatty BG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.18 (meprin A)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Chromosomes, Human/*genetics
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 13/genetics
MH  - Chromosomes, Human, Pair 6/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Cloning, Molecular
MH  - Gene Dosage
MH  - Genes/*genetics
MH  - Genome, Human
MH  - Genomic Library
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Metalloendopeptidases/chemistry/*genetics
MH  - Mice
MH  - *Sequence Homology, Nucleic Acid
EDAT- 1999/07/16 00:00
MHDA- 1999/07/16 00:01
CRDT- 1999/07/16 00:00
PHST- 1999/07/16 00:00 [pubmed]
PHST- 1999/07/16 00:01 [medline]
PHST- 1999/07/16 00:00 [entrez]
AID - S0006-291X(99)91008-2 [pii]
AID - 10.1006/bbrc.1999.1008 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Jul 22;261(1):163-8. doi: 
      10.1006/bbrc.1999.1008.