PMID- 10405758
OWN - NLM
STAT- MEDLINE
DCOM- 19990927
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 11
IP  - 7
DP  - 1999 Jul
TI  - Dual activity of pyrrolidine dithiocarbamate on kappa B-dependent gene expression 
      in U937 cells: I. Regulation by the phorbol ester TPA.
PG  - 479-89
AB  - Pyrrolidine dithiocarbamate (PDTC) has been widely used as an inhibitor of the 
      nuclear factor-kappa B, (NF-kappa B) signalling pathway. Here, we show that kappa 
      B-dependent reporter gene expression induced by low concentrations of 
      12-O-tetradecanoylphorbol-13-acetate (TPA) is potentiated by PDTC in the human 
      pro-monocytic U937 cell line. The stimulatory effect of PDTC on kappa B-dependent 
      gene expression was shown with a 4 x kappa B chloramphenicol acetyltransferase 
      construct and required an intact kappa B element in the human immunodeficiency 
      virus long terminal repeat (HIV-1 LTR). Unexpectedly, an HIV-1 LTR construct with 
      a mutation of the activator protein 2 (AP-2) binding site located between the two 
      kappa B elements was unresponsive to the stimulatory effect of PDTC with TPA. The 
      stimulation or inhibition of kappa B-dependent gene expression was dependent on 
      PDTC pre-treatment and the concentration of TPA. No stimulatory effect on HIV-1 
      LTR activity was observed with the metal chelator dipyridyl or the anti-oxidant 
      N-acetyl-L-cysteine. These results are consistent with the hypothesis that PDTC 
      treatment potentiated kappa B-dependent gene expression in a manner dependent on 
      the concentration of TPA.
FAU - Watanabe, K
AU  - Watanabe K
AD  - Signal Transduction Laboratory, SRI International, Menlo Park, CA 94025, USA.
FAU - Kazakova, I
AU  - Kazakova I
FAU - Furniss, M
AU  - Furniss M
FAU - Miller, S C
AU  - Miller SC
LA  - eng
GR  - GM48229/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Chelating Agents)
RN  - 0 (Metals)
RN  - 0 (NF-kappa B)
RN  - 0 (Pyrrolidines)
RN  - 0 (Thiocarbamates)
RN  - 0 (Transcription Factor AP-1)
RN  - 25769-03-3 (pyrrolidine dithiocarbamic acid)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Chelating Agents
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - Gene Expression Regulation
MH  - *Gene Expression Regulation, Viral
MH  - Genes, Reporter
MH  - *HIV Long Terminal Repeat
MH  - HIV-1/*genetics
MH  - Humans
MH  - Metals
MH  - NF-kappa B/genetics/*metabolism
MH  - Pyrrolidines/*metabolism/pharmacology
MH  - Tetradecanoylphorbol Acetate/*metabolism/pharmacology
MH  - Thiocarbamates/*metabolism/pharmacology
MH  - Time Factors
MH  - Transcription Factor AP-1/metabolism
MH  - U937 Cells
EDAT- 1999/07/16 00:00
MHDA- 1999/07/16 00:01
CRDT- 1999/07/16 00:00
PHST- 1999/07/16 00:00 [pubmed]
PHST- 1999/07/16 00:01 [medline]
PHST- 1999/07/16 00:00 [entrez]
AID - S0898-6568(99)00023-6 [pii]
AID - 10.1016/s0898-6568(99)00023-6 [doi]
PST - ppublish
SO  - Cell Signal. 1999 Jul;11(7):479-89. doi: 10.1016/s0898-6568(99)00023-6.